Genetic architecture of fatty acid composition in the longissimus dorsi muscle revealed by genome-wide association studies on diverse pig populations.
Genetic architecture of fatty acid composition in the longissimus dorsi muscle revealed by genome-wide association studies on diverse pig populations.
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通过对不同猪群的全基因组关联研究揭示背最长肌脂肪酸组成的遗传结构
DOI：
10.1186/s12711-016-0184-2
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发表时间：
2016-01-21
期刊：
影响因子：
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通讯作者：
Yang B
中科院分区：
文献类型：
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作者：
Zhang W;Zhang J;Cui L;Ma J;Chen C;Ai H;Xie X;Li L;Xiao S;Huang L;Ren J;Yang B
肌肉中的脂肪酸组成是影响猪肉营养价值和口感的重要因素。为了研究猪肉脂肪酸组成的遗传结构，我们测量了来自三个不同群体的1244头猪背最长肌的脂肪酸含量，并对脂肪酸含量进行了全基因组关联研究（GWAS）。在8条染色体（Sus scrofa为SSC）上检测到8种脂肪酸的26个全基因组显著数量性状位点（QTL）。这些位点不仅复制了先前报道的SSC14上的C18:0和SSC16上的C20:0的QTL，而且还包含了SSC7上的C20:1、SSC9上的C14:0和SSC12上的C14:0、C16:0和C16:1的QTL。此外，我们对五个人群进行了GWAS的荟萃分析，包括本研究中调查的三个人群和我们之前研究过的另外两个人群。这增强了脂肪酸组成与几个标记位点之间的关联强度，特别是SSC14上的C18:0和SSC16上的C20:0。与脂肪酸代谢直接相关的基因ELOVL5、ELOVL6、ELOVL7、FASN、SCD和THRSP在6个显著QTL中最接近顶端相关标记。这些发现提高了我们对猪肉脂肪酸组成的遗传结构的理解，并有助于进一步精细绘制和表征影响脂肪酸组成的基因。本文的在线版本（doi:10.1186/s12711-016-0184-2）包含补充材料，可供授权用户使用。
Fatty acid composition in muscle is an important factor that affects the nutritive value and taste of pork. To investigate the genetic architecture of fatty acid composition of pork, we measured fatty acid contents in longissimus dorsi muscle of 1244 pigs from three divergent populations and conducted genome-wide association studies (GWAS) for fatty acid contents. We detected 26 genome-wide significant quantitative trait loci (QTL) on eight chromosomes (SSC for Sus scrofa) for eight fatty acids. These loci not only replicated previously reported QTL for C18:0 on SSC14 and C20:0 on SSC16, but also included several novel QTL such as those for C20:1 on SSC7, C14:0 on SSC9, and C14:0, C16:0 and C16:1 on SSC12. Furthermore, we performed a meta-analysis of GWAS on five populations, including the three populations that were investigated in this study and two additional populations that we had previously examined. This enhanced the strength of the associations detected between fatty acid composition and several marker loci, especially for those for C18:0 on SSC14 and C20:0 on SSC16. The genes ELOVL5, ELOVL6, ELOVL7, FASN, SCD and THRSP, which have functions that are directly relevant to fatty acid metabolism, are proximal to the top associated markers at six significant QTL. The findings improve our understanding of the genetic architecture of fatty acid composition in pork and contribute to further fine-map and characterize genes that influence fatty acid composition. The online version of this article (doi:10.1186/s12711-016-0184-2) contains supplementary material, which is available to authorized users.