Chemomics-Integrated Proteomics Analysis of Jie-Geng-Tang to Ameliorate Lipopolysaccharide-Induced Acute Lung Injury in Mice.
Chemomics-Integrated Proteomics Analysis of Jie-Geng-Tang to Ameliorate Lipopolysaccharide-Induced Acute Lung Injury in Mice.
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结庚汤改善脂多糖诱导的小鼠急性肺损伤的化学组学-蛋白质组学分析
DOI：
10.1155/2016/7379146
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Bai G
中科院分区：
文献类型：
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作者：
Tao J;Nie Y;Hou Y;Ma X;Ding G;Gao J;Jiang M;Bai G
桔梗汤是以桔梗（Platycodongrandiflorum（Jacq.）A. DC. (PG)和乌拉尔甘草菲施。(GU)，以“清热解毒”和“宣肺利咽”而闻名。然而，其作用机制仍不清楚。本研究筛选了潜在的抗炎活性成分，并提交到PharmMapper和KEGG生物信息学网站，分别预测靶蛋白和相关通路。通过用于相对和绝对定量（iTRAQ）的同量异位素标签和LC Triple-TOF鉴定来自用JGT治疗的急性肺损伤（ALI）小鼠的差异表达的候选蛋白。发现了11种潜在的抗炎成分，包括甘草酸、甘草皂苷、甘草苷和桔梗皂苷元的衍生物。JGT治疗后，共发现67个差异表达蛋白，具有免疫调节、抗炎、核糖体和肌肉收缩等4种治疗功能。PG和GU可协同PI 3 K/Akt信号通路抑制NF-κB、VCAM 1和ICAM 1的释放，这些信号通路主要作用于PI 3 K、PDK 1、AKT和GSK 3 β。GU显著抑制ERK/MAPK信号通路，主要作用于LCK、RAS和MEK。利用生物活性成分、靶点和途径构建了一个网络，以确定JGT治疗ALI的机制。
Jie-Geng-Tang (JGT), a classic and famous traditional Chinese medicine (TCM) prescription composed of Platycodon grandiflorum (Jacq.) A. DC. (PG) and Glycyrrhiza uralensis Fisch. (GU), is well known for “clearing heat and relieving toxicity” and its ability to “diffuse the lung and relieve sore throat.” However, the mechanism underlying its action remains unclear. In this study, potential anti-inflammatory ingredients were screened and submitted to PharmMapper and the KEGG bioinformatics website to predict the target proteins and related pathways, respectively. Differentially expressed candidate proteins from acute lung injury (ALI) mice treated with JGT were identified by isobaric tags for relative and absolute quantitation (iTRAQ) and LC Triple-TOF. Eleven potential anti-inflammatory ingredients were found, including the derivatives of glycyrrhizic acid, licorice-saponin, liquiritin, and platycodigenin. A total of sixty-seven differentially expressed proteins were confirmed after JGT treatment with four therapeutic functions, including immunoregulation, anti-inflammation, ribosome, and muscle contraction. PG and GU comediate PI3K/Akt signal pathway inhibition of NF-κB, VCAM1, and ICAM1 release which primarily act on PI3K, PDK1, AKT, and GSK3β. GU markedly inhibits the ERK/MAPK signaling pathways and primarily acts on LCK, RAS, and MEK. A network was constructed using bioactive ingredients, targets, and pathways to determine the mechanism underlying JGT treatment of ALI.