Effects of multi-walled carbon nanotubes on a murine allergic airway inflammation model
Effects of multi-walled carbon nanotubes on a murine allergic airway inflammation model
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DOI：
10.1016/j.taap.2009.04.003
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发表时间：
2009-06-15
影响因子：
3.8
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
--
作者：
Inoue, Ken-ichiro;Koike, Eiko;Takano, Hirohisa
纳米技术的发展增加了暴露于环境中燃烧产生的颗粒以外的各种颗粒（即工业纳米材料）的风险。另一方面，支气管哮喘患者对吸入颗粒物等物质敏感。本研究探讨了肺部暴露于一种纳米尺寸的碳纳米管（多壁纳米管：MWCNT）对体内变应性气道炎症的影响及其体外细胞机制。在体内将ICR小鼠分为4个实验组。小鼠、MWCNT （50 μ g/只）、卵清蛋白（OVA）和OVA + MWCNT气管内重复给药。研究支气管肺泡灌洗（BAL）细胞结构、肺组织学、肺匀浆/BAL液（BALF's）中与过敏性炎症相关的细胞因子水平以及血清免疫球蛋白水平。此外，我们还评估了MWCNT （0.1-1 μ g/ml）对体外骨髓源性树突状细胞（DC）表型和功能的影响。MWCNT加重了过敏原诱导的气道炎症，其特征是肺中嗜酸性粒细胞、中性粒细胞和单核细胞的浸润，以及支气管上皮中杯状细胞数量的增加。与单独的过敏原相比，MWCNT与过敏原相结合可扩增Th细胞因子和趋化因子的肺蛋白水平。MWCNT对过敏原特异性IgG(1)和IgE表现出佐剂活性。MWCNT显著增加了过敏原（OVA）特异性同基因t细胞的增殖，特别是在体外较低浓度下。综上所述，MWCNT可以通过促进th优势环境，至少部分地加剧小鼠过敏性气道炎症。此外，恶化可能部分是由于抗原呈递细胞（包括DC）的不适当激活。(C) 2009爱思唯尔公司版权所有。
The development of nanotechnology has increased the risk of exposure to types of particles other than combustion-derived particles in the environment, namely, industrial nanomaterials. On the other hand, patients with bronchial asthma are sensitive to inhaled substances including particulate matters. This Study examined the effects of pulmonary exposure to a type of nano-sized carbon nanotube (multi-walled nanotubes: MWCNT) on allergic airway inflammation in vivo and their cellular mechanisms in vitro. In vivo, ICR mice were divided into 4 experimental groups. Vehicle, MWCNT (50 mu g/animal), ovalbumin (OVA), and OVA + MWCNT were repeatedly administered intratracheally. Bronchoalveolar lavage (BAL) cellularity, lung histology, levels of cytokines related to allergic inflammation in lung homogenates/BAL fluids (BALF's), and serum immunoglobulin levels were Studied. Also, we evaluated the impact of MWCNT (0.1-1 mu g/ml) on the phenotype and function of bone marrow-derived dendritic cells (DC) in vitro. MWCNT aggravated allergen-induced airway inflammation characterized by the infiltration of eosinophils, neutrophils, and mononuclear cells in the lung, and an increase in the number of goblet cells in the bronchial epithelium. MWCNT with allergen amplified lung protein levels of Th cytokines and chemokines compared with allergen alone. MWCNT exhibited adjuvant activity for allergen-specific IgG(1) and IgE. MWCNT significantly increased allergen (OVA)-specific syngeneic T-cell proliferation, particularly at a lower concentration in vitro. Taken together, MWCNT can exacerbate murine allergic airway inflammation, at least partly, via the promotion of a Th-dominant milieu. In addition, the exacerbation may be partly through the inappropriate activation of antigen-presenting cells including DC. (C) 2009 Elsevier Inc. All rights reserved.