Viral Co-Infections in Pediatric Patients Hospitalized with Lower Tract Acute Respiratory Infections.
Viral Co-Infections in Pediatric Patients Hospitalized with Lower Tract Acute Respiratory Infections.
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DOI：
10.1371/journal.pone.0136526
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
GENDRES network
中科院分区：
文献类型：
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作者：
Cebey-López M;Herberg J;Pardo-Seco J;Gómez-Carballa A;Martinón-Torres N;Salas A;Martinón-Sánchez JM;Gormley S;Sumner E;Fink C;Martinón-Torres F;GENDRES network
分子技术通常可以揭示呼吸道感染中更广泛的病原体。我们的目的是利用分子生物学技术，调查因下呼吸道急性呼吸道感染（LT-ARI）住院的儿童中病毒合并感染的患病率和年龄模式。使用巢式聚合酶链反应方法检测2011-2013年期间在任何GENDRES网络医院前瞻性收治的急性呼吸道感染儿童的呼吸道样本中的流感病毒（A、B）、偏肺病毒、呼吸道合胞病毒（RSV）、副流感病毒（1-4）、鼻病毒、腺病毒（A-F）、博卡病毒和冠状病毒（NL 63、229 E、OC 43）。在英国收集的一个独立队列中证实了这一结果。在GENDRES和UK队列中分别采集了总计204份和97份鼻咽样本。在两个队列中，RSV是最常见的病原体（分别占队列的52.9%和36.1%）。分别在92个样本（45.1%）和29个样本（29.9%）中发现了多种病毒的合并感染;这在12-24个月年龄组中最常见。最常见的合并感染模式为RSV-鼻病毒（23例患者，11.3%，GENDRES队列）和RSV-博卡病毒/博卡病毒-流感（5例患者，5.2%，英国队列）。在因LT-ARI入院的儿科患者中，一种以上病毒的存在非常常见，似乎在12-24个月大时达到高峰。这些发现的临床意义尚不清楚，但应进一步分析。
Molecular techniques can often reveal a broader range of pathogens in respiratory infections. We aim to investigate the prevalence and age pattern of viral co-infection in children hospitalized with lower tract acute respiratory infection (LT-ARI), using molecular techniques. A nested polymerase chain reaction approach was used to detect Influenza (A, B), metapneumovirus, respiratory syncytial virus (RSV), parainfluenza (1–4), rhinovirus, adenovirus (A—F), bocavirus and coronaviruses (NL63, 229E, OC43) in respiratory samples of children with acute respiratory infection prospectively admitted to any of the GENDRES network hospitals between 2011–2013. The results were corroborated in an independent cohort collected in the UK. A total of 204 and 97 nasopharyngeal samples were collected in the GENDRES and UK cohorts, respectively. In both cohorts, RSV was the most frequent pathogen (52.9% and 36.1% of the cohorts, respectively). Co-infection with multiple viruses was found in 92 samples (45.1%) and 29 samples (29.9%), respectively; this was most frequent in the 12–24 months age group. The most frequently observed co-infection patterns were RSV—Rhinovirus (23 patients, 11.3%, GENDRES cohort) and RSV—bocavirus / bocavirus—influenza (5 patients, 5.2%, UK cohort). The presence of more than one virus in pediatric patients admitted to hospital with LT-ARI is very frequent and seems to peak at 12–24 months of age. The clinical significance of these findings is unclear but should warrant further analysis.