3D bioprinted graphene oxide-incorporated matrix for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells
3D bioprinted graphene oxide-incorporated matrix for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells
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DOI：
10.1016/j.carbon.2017.02.049
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发表时间：
2017-05-01
期刊：
影响因子：
10.9
通讯作者：
Zhang, Lijie Grace
中科院分区：
文献类型：
--
作者：
Zhou, Xuan;Nowicki, Margaret;Zhang, Lijie Grace
由于软骨组织固有的再生能力非常弱，关节软骨的修复和再生是一个世界性的挑战性问题。三维生物打印作为一种新兴的组织工程支架制作技术，在制造具有层次化结构的可定制人工组织基质方面显示出巨大的应用前景。本研究的目的是研究三维生物印染的氧化石墨烯(GO)掺杂明胶支架促进人骨髓间充质干细胞(MSCs)向软骨方向分化。本研究制备了聚乙二醇二丙烯酸酯(PEGDA)-甲基丙烯酸酯明胶(GeIMA)，作为一种生物相容性的光聚合生物墨水。将多功能碳基纳米材料GO引入生物墨水中，用于促进软骨细胞的分化。最后，利用该新型台式立体光刻机制作了具有层次化结构的3D打印GeIMA-PEGDA-GO支架。结果表明，GeIMA-PEGDA-GO支架可显著提高GO诱导的hMSCs向软骨分化后的糖胺聚糖和胶原水平。此外，与软骨形成相关的胶原II、Sox 9和Aggrecan基因的表达在支架上也得到了极大的促进。本研究表明，可定制的3D打印GeIMA-PEGDA-GO支架是促进hMSCs向软骨细胞分化的极佳候选材料，因此有望成为未来软骨再生医学应用的候选材料。(C)2017爱思唯尔有限公司。保留所有权利。
Articular cartilage repair and regeneration are a challenging problem worldwide due to the extremely weak inherent regenerative capacity of cartilaginous tissue. As an emerging tissue engineering scaffold fabrication technology, 3D bioprinting has shown great promise in fabricating customizable artificial tissue matrices with hierarchical structures. The goal of the present study is to investigate 3D bioprinted graphene oxide (GO)-doped gelatin based scaffolds for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). In the current study, GO-gelatin methacrylate (GeIMA) poly (ethylene glycol) diacrylate (PEGDA) was prepared as a biocompatible photopolymerizable bioink. GO, a multifunctional carbon based nanomaterial, was incorporated into the bioink for promoting chondrogenic differentiation. Finally, the 3D printed GeIMA-PEGDA-GO scaffold with hierarchical structures was fabricated via our novel table-top stereolithography-based printer. Results showed that GeIMA-PEGDA-GO scaffolds greatly promoted the glycosaminoglycan, and collagen levels after GO induced chondrogenic differentiation of hMSCs. Moreover, the Collagen II, SOX 9, and Aggrecan gene expressions associated with chondrogenesis were greatly promoted on the scaffolds. This study demonstrated that customizable 3D printed GeIMA-PEGDA-GO scaffolds are excellent candidates for promoting chondrogenic differentiation of hMSCs and are therefore promising candidates for future cartilage regenerative medicine applications. (C) 2017 Elsevier Ltd. All rights reserved.