A Lean Laboratory Operational Simplicity and Cost Effectiveness of the Luminex xTAG™ Respiratory Viral Panel
A Lean Laboratory Operational Simplicity and Cost Effectiveness of the Luminex xTAG™ Respiratory Viral Panel
复制标题
DOI：
10.1016/j.jmoldx.2010.09.003
复制
发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Rogers, Beverly B.
中科院分区：
文献类型：
--
作者：
Dundas, Nicola E.;Ziadie, Mandolin S.;Rogers, Beverly B.
在一年中的某些月份，病毒性呼吸道感染导致儿科急诊室就诊和住院人数急剧增加。快速识别传染性微生物可以及时治疗并减少住院费用和住院时间。在病毒学实验室引入分子检测之前，诊断依赖于免疫荧光和培养的标准方法。这些测试可能是劳动密集型且成本高昂的。最近的研究表明，多重 RT-PCR 检测具有更高的灵敏度、更快的周转时间和更广泛的诊断谱。缺乏比较实验室成本和测试劳动效率的数据。为了解决这个问题，我们选择使用精益方法来实施操作工作流程分析的原则，以批判性地评估多重 RT-PCR 检测在工作流程和成本效益方面的潜在优势。我们的结果表明，Luminex xTAG 呼吸道病毒检测试剂盒 (RVP) 的实施实现了标准化工作流程，降低了实验室成本要求并提高了效率。总之，我们证明，在我们的实验室中，Luminex xTAG RVP 比标准病毒直接荧光抗体和培养物在操作上更加简化且更具成本效益。需要进一步的研究来强调该测试的其他好处，包括缩短住院时间和改善患者的治疗结果。 (J Mol Dingo 2011, 13: 175-179; DOI: 10.1016/j.jmoldx.2010.09.003)
During certain months of the year, viral respiratory infections lead to a dramatic increase in pediatric emergency room visits and hospital admissions. Rapid identification of the infectious organism results in timely treatment and reductions in hospital cost and length of stay. Before the introduction of molecular testing to the virology laboratory, diagnosis relied on the standard methods of immunofluorescence and culture. These tests can be labor-intensive and costly. Recent studies have demonstrated the higher sensitivity, faster turnaround, and broader diagnostic spectrum provided by multiplexed RT-PCR assays. Data comparing the laboratory cost and labor efficiency of the tests are lacking. To address this issue, we chose to implement the principles of operational workflow analysis using lean methodology to critically evaluate the potential advantages of a multiplexed RT-PCR assay both in terms of workflow and cost effectiveness. Our results indicated that the implementation of the Luminex xTAG Respiratory Viral Panel (RVP) resulted in a standardized workflow with decreased requirements in laboratory cost as well as improvement in efficiency. In summary, we demonstrate that, in our laboratory, the Luminex xTAG RVP is more operationally streamlined and cost-effective than standard viral direct fluorescent antibody and culture. Further studies are needed to highlight additional benefits of the test, including shortened hospital stay and improved patient outcome. (J Mol Dingo 2011, 13: 175-179; DOI: 10.1016/j.jmoldx.2010.09.003)