The Manipulation of RNA-Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
The Manipulation of RNA-Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
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利用茚三酮化学操作 RNA 引导核酸切割
DOI：
10.1002/advs.201903770
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发表时间：
2020
期刊：
影响因子：
15.1
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Wang Shao-Ru;Huang Hai-Yan;Liu Jian;Wei Lai;Wu Ling-Yu;Xiong Wei;Yin Ping;Tian Tian;Zhou Xiang
CRISPR（成簇的规则间隔短回文重复序列）系统已被确立为有价值的基因组编辑工具。控制CRISPR系统具有很高的生物学意义，这一领域引起了人们的浓厚兴趣。有一个相当大的需要简单的方法，而不需要蛋白质工程。CRISPR系统通常需要引导RNA（gRNA）部分来募集和引导核酸酶复合物。在这方面，茚三酮（1，2，3-茚三酮一水合物）似乎具有相当大的潜力，但尚未开发，用于修饰gRNA。在本研究中，探索了茚三酮化学用于gRNA分子的可逆合成后修饰。进一步表明，茚三酮化学在调节两个重要的CRISPR系统方面是有效的。因此，茚三酮化学在未来的化学生物学研究中具有潜在的应用。
CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome‐editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need for protein engineering. The CRISPR systems usually require a guide RNA (gRNA) moiety to recruit and direct the nuclease complexes. In this respect, the ninhydrin (1,2,3‐indantrione monohydrate) seems to have considerable potential, as yet unexploited, for modifying gRNA. In this study, ninhydrin chemistry is explored for reversible postsynthetic modification of gRNA molecules. It is further shown that ninhydrin chemistry is efficient in modulating two important CRISPR systems. Thus, ninhydrin chemistry exhibits potential applications in future chemical biology studies.