Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos
Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos
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DOI：
10.1006/dbio.2002.0641
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发表时间：
2002-05-01
影响因子：
2.7
通讯作者：
Lumsden, A
中科院分区：
文献类型：
--
作者：
Chapman, SC;Schubert, FR;Lumsden, A
对吻尾规范、神经诱导和头部发育的遗传基础的研究需要了解相关的基因表达模式。我们对基因表达的理解存在差距，这使得我们研究了与早期发育有关的19个基因的详细时空表达模式，以了解它们在指定和构建导致头部形成的早期发育过程中的潜在作用。在这里，我们报告了这些标记在囊胚期和原肠胚期鸡胚胎中的表达模式，采用整体原位杂交。Nodeal、Fgf8、Bmp7、Chordin、Lim1、Hnf3 beta、OTX2、Goosecid、Cerberus、Hex、Dickkopf1和新月在产卵时都已经表达。当原始条纹达到其全长时，随后的一组基因开始表达，包括Ganf、Six3、BMP2、Bmp4、Noggin、Follistatin和Qin(BF1)。基于对这些动态时空表达模式的分析，我们重新评估了当前的早期吻部模式模型。(C)2002年埃尔塞维尔科学公司(美国)。
Studies on the genetic basis of rostral-caudal specification, neural induction, and head development require knowledge of the relevant gene expression patterns. Gaps in our understanding of gene expression have led us to examine the detailed spatiotemporal expression patterns of 19 genes implicated in early development, to learn more about their potential role in specifying and patterning early developmental processes leading to head formation. Here, we report the expression patterns of these markers in blastula- and gastrula-stage chick embryos, using whole-mount in situ hybridisation. Nodal, Fgf8, Bmp7, Chordin, Lim1, Hnf3beta, Otx2, Goosecoid, Cerberus, Hex, Dickkopf1, and Crescent are all already expressed by the time the egg is laid. When the primitive streak has reached its full length, a later group of genes, including Ganf, Six3, Bmp2, Bmp4, Noggin, Follistatin, and Qin (BF1), begins to be expressed. We reassess current models of early rostral patterning based on the analysis of these dynamic spatiotemporal expression patterns. (C) 2002 Elsevier Science (USA).