Structural similarities between topoisomerases that cleave one or both DNA strands
Structural similarities between topoisomerases that cleave one or both DNA strands
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DOI：
10.1073/pnas.95.14.7876
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发表时间：
1998-07-07
影响因子：
11.1
通讯作者：
Harrison, SC
中科院分区：
文献类型：
--
作者：
Berger, JM;Fass, D;Harrison, SC
IA型和II型DNA拓扑异构酶的区别在于它们能够分别切割DNA双链的一条或两条链。这两种类型都被提议使用“酶桥接”机制，在DNA链中形成断裂，断裂片段之间打开间隙，允许第二DNA链或双链片段通过。尽管IA型和II型拓扑异构酶的结构看起来完全不同，但在几个结构元件之间意想不到的相似性表明，这两个亚家族的成员可能使用类似的机制来结合和切割DNA。
Type IA and type II DNA topoisomerases are distinguished by their ability to cleave one or two strands, respectively, of a DNA duplex. Both types have been proposed to use an "enzyme-bridging" mechanism, in which a break is formed in a DNA strand and a gap is opened between the broken pieces to allow passage of a second DNA strand or duplex segment. Although the type IA and type II topoisomerase structures appear overall quite different from one another, unexpected similarities between several structural elements suggest that members of the two subfamilies may use comparable mechanisms to bind and cleave DNA.