Characterization of human maligant melanoma cell lines. I. Morphology and growth characteristics in culture.
Characterization of human maligant melanoma cell lines. I. Morphology and growth characteristics in culture.
复制标题
人类恶性黑色素瘤细胞系的表征。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
P. McCulloch
中科院分区：
文献类型：
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作者：
S. Liao;P. Dent;P. McCulloch
将7株人恶性黑色素瘤细胞系在体外培养不同时间。本实验室从转移性实体肿瘤用0.02%EDTA反复处理原发肿瘤而建立的一种系，允许无成纤维细胞的黑色素瘤细胞的连续培养。光镜下，每株细胞可分为三种形态类型：细长树突状、立方形或三角形树突状。7个品系中有4个表现出不同程度的色素沉着。每条线的电镀效率和饱和密度决定了生长模式。用荧光显带技术进行的细胞遗传学分析显示，只有人类染色体具有粗大的非整倍体。确定了每个品系所特有的主要标记染色体。除了细长的枝晶形态与较差的镀膜效率和较低的饱和密度有关外，所有研究参数都没有显示出任何相互关联或相互依赖。
Seven human malignant melanoma lines were maintained in vitro for various periods of time. One line, established in this laboratory from a metastatic solid tumor by repeated treatment of the primary outgrowth with 0.02 percent EDTA, allowed a continuous culture of melanoma cells free of fibroblasts. By light microscopy, cells in each line could be classified into one of three morphologic types: elongated dendritic, cuboidal, or triangular dendritic. Four of the seven lines exhibited various degrees of pigmentation. The growth pattern was determined by plating efficiency and saturation density for each line. Cytogenetic analysis with the fluorescent banding technique revealed only human chromosomes with gross aneuploidy. Major marker chromosomes specific for each line were identified. None of the parameters studied showed any correlation or interdependence with one another, except for an association of elongated dendritic morphology with poor plating efficiency and low saturation density.