Phylogenetic Analysis of Uromyces Species Infecting Grain and Forage Legumes by Sequence analysis of Nuclear Ribosomal Internal Transcribed Spacer Region
Phylogenetic Analysis of Uromyces Species Infecting Grain and Forage Legumes by Sequence analysis of Nuclear Ribosomal Internal Transcribed Spacer Region
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DOI：
10.1111/j.1439-0434.2010.01736.x
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发表时间：
2011-03-01
影响因子：
1.5
通讯作者：
Torres, Ana M.
中科院分区：
文献类型：
--
作者：
Barilli, Eleonora;Satovic, Zlatko;Torres, Ana M.
对核核糖体内转录间隔区 (ITS) 进行 DNA 序列分析，以确定感染谷物和饲料豆类的 49 个锈菌分离株之间的系统发育关系。分离物是从不同宿主和不同地理来源收集的，代表八种尿霉菌：U. anthyllidis、U.appendiculatus、U. ciceris-arietini、U.minor、U.pisi、U.striatus、U.viciae-fabae和U.vignae。 ITS 序列揭示了 DNA 序列的长度多态性和变异，用于通过最大简约性、最大似然性和贝叶斯分析来表征系统发育关系，这些分析普遍同意揭示了四个明显不同的进化枝的存在。第一分支包括引起鹰嘴豆、胡芦巴和苜蓿锈病的分离株。第二个进化枝由田三叶草和豌豆植物的锈菌分离物组成，而第三个进化枝由菜豆和豇豆分离物形成。第四支是最大的，包括感染蚕豆的所有锈菌分离株。在这个分支中，在Lens culinaris上收集的U. viciae-fabae、在Vicia sativa上收集的U. viciae-fabae和在沼黧豆上收集的U. viciae-fabae得到高度支持的亚群表明宿主专业化的持续过程。
DNA sequence analysis of the nuclear ribosomal internal transcribed spacer region (ITS) was performed to determine phylogenetic relationship between 49 isolates of rusts infecting grain and forage legumes. Isolates were collected from different hosts and distinct geographic origins and represent eight species of Uromyces: U. anthyllidis, U. appendiculatus, U. ciceris-arietini, U. minor, U. pisi, U. striatus, U. viciae-fabae and U. vignae. ITS sequences revealed length polymorphisms and variation in DNA sequence that were used to characterize phylogenetic relationships by maximum parsimony, maximum likelihood and Bayesian analyses which in general agreed revealing the presence of four clearly distinct clades. Clade one included the isolates causing rust on chickpea, fenugreek and alfalfa. Clade two was composed by rust isolates of field clover and pea plants, while the third clade was formed by bean and cowpea isolates. Clade four was the largest and included all the rust isolates infecting faba bean. Within this clade, the highly supported subclusters of U. viciae-fabae collected on Lens culinaris, U. viciae-fabae collected on Vicia sativa and U. viciae-fabae collected on Lathyrus palustris suggest an ongoing process of host specialization.