IGF-I IS REQUIRED FOR NORMAL EMBRYONIC GROWTH IN MICE
IGF-I IS REQUIRED FOR NORMAL EMBRYONIC GROWTH IN MICE
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DOI：
10.1101/gad.7.12b.2609
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发表时间：
1993-12-01
影响因子：
10.5
通讯作者：
STEWART, TA
中科院分区：
文献类型：
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作者：
POWELLBRAXTON, L;HOLLINGSHEAD, P;STEWART, TA
IGF-I是一种多效性激素，据报道可影响线性生长、葡萄糖代谢、器官稳态以及免疫和神经系统。与IGF-II相比，IGF-I在胚胎时期表达水平较低，并且被认为对出生后的生长和发育更为重要。为了研究IGF-I在正常发育中的作用，我们在胚胎干细胞中通过同源重组制备了IGF-I基因失活的小鼠。杂合子小鼠健康且可生育，但它们比野生型幼鼠小10-20%，igf - 1水平低于正常水平。体型减小的原因是器官、肌肉和骨量的减少。然而，所有组织在组织学上都是正常的。出生时纯合突变小鼠（IGF-I-/-）
IGF-I is a pleiotropic hormone reported to affect linear growth, glucose metabolism, organ homeostasis, and the immune and neurologic systems. In contrast to IGF-II, IGF-I is expressed at low levels embryonically and has been thought to be more important for postnatal growth and development. To investigate the role of IGF-I in normal development we generated mice with an inactive IGF-I gene by homologous recombination in ES cells. Heterozygous mice are healthy and fertile, but they are 10-20% smaller than wild-type littermates and have lower than normal levels of IGF-I. The size reduction is attributable to a decrease in organs and muscle and bone mass. However, all tissues appear histologically normal. At birth homozygous mutant mice (IGF-I-/-) are