Role of lineage-specific matrix in stem cell chondrogenesis
Role of lineage-specific matrix in stem cell chondrogenesis
复制标题
DOI：
10.1016/j.biomaterials.2019.119681
复制
发表时间：
2020-02-01
期刊：
影响因子：
14
通讯作者：
Pei, Ming
中科院分区：
文献类型：
--
作者：
Li, Jingting;Narayanan, Karthikeyan;Pei, Ming
由于软骨的再生能力有限，临床上的软骨修复是一个挑战。滑膜源性干细胞（SDSC）被认为是软骨形成的组织特异性干细胞。在这项研究中，我们假设SDSC沉积的脱细胞细胞外基质（dECM）可以提供一个上级组织特异性基质微环境，用于软骨再生的成体SDSC的最佳复壮。dECM由具有不同软骨形成能力的成体干细胞沉积; SDSC（强）（SECM）、脂肪来源的干细胞（弱）（AECM）和真皮成纤维细胞（弱）（DECM）以及尿来源的干细胞（无）（UECM）。使用塑料烧瓶（塑料）作为对照底物。将人SDSC在上述基质上扩增一次，并检查软骨形成能力。我们发现每个dECM由独特的基质蛋白组成，并表现出不同的刚度，这影响了细胞的形态和弹性。在dECM上生长的人SDSC显示出细胞增殖的显著增加和独特的表面表型。在诱导培养基下，dECM扩增的细胞产生具有显著增加的软骨形成标志物数量的团块。有趣的是，SECM扩增的细胞与在其他dECM上生长的细胞相比具有较小的肥大潜力，表明组织特异性基质可能为干细胞软骨形成分化提供上级微环境。
Cartilage repair in clinics is a challenge owing to the limited regenerative capacities of cartilage. Synovium-derived stem cells (SDSCs) are suggested as tissue-specific stem cells for chondrogenesis. In this study, we hypothesize that decellularized extracellular matrix (dECM) deposited by SDSCs could provide a superior tissue-specific matrix microenvironment for optimal rejuvenation of adult SDSCs for cartilage regeneration. dECMs were deposited by adult stem cells with varying chondrogenic capacities; SDSCs (strong) (SECM), adipose-derived stem cells (weak) (AECM) and dermal fibroblasts (weak) (DECM), and urine-derived stem cells (none) (UECM). Plastic flasks (Plastic) were used as a control substrate. Human SDSCs were expanded on the above substrates for one passage and examined for chondrogenic capacities. We found that each dECM consisted of unique matrix proteins and exhibited varied stiffnesses, which affected cell morphology and elasticity. Human SDSCs grown on dECMs displayed a significant increase in cell proliferation and unique surface phenotypes. Under induction media, dECM expanded cells yielded pellets with a dramatically increased number of chondrogenic markers. Interestingly, SECM expanded cells had less potential for hypertrophy compared to those grown on other dECMs, indicating that a tissue-specific matrix might provide a superior microenvironment for stem cell chondrogenic differentiation.