Global gene expression profiles of the cyanobacterium Synechocystis sp strain PCC 6803 in response to irradiation with UV-B and white light
Global gene expression profiles of the cyanobacterium Synechocystis sp strain PCC 6803 in response to irradiation with UV-B and white light
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DOI：
10.1128/jb.184.24.6845-6858.2002
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发表时间：
2002-12-01
影响因子：
3.2
通讯作者：
LaRossa, RA
中科院分区：
文献类型：
--
作者：
Huang, LX;McCluskey, MP;LaRossa, RA
我们建立了一种转录谱方法来阐明蓝藻Synehoeystis sp.的表达模式。菌株PCC 6803，并使用该技术研究了中波长紫外线(TN-B)或高强度白光照射引起的基因表达变化。UV-B处理改变了几个转录本家族，包括指定参与光收集、光合作用、光保护和热休克反应的蛋白质的mRNAs。此外，UV-B光诱导了集胞藻的严格反应，如核糖体蛋白转录本和其他参与翻译的mRNAs的抑制。高强度白光和UV-B介导的表达谱在光合作用基因的下调和热休克反应的诱导方面重叠，但在其他几个转录过程中不同，包括那些指定二氧化碳吸收和固定的转录过程，严格的反应，以及高光诱导蛋白的诱导谱。这两个图谱的比较不仅证实了已知的生理变化，而且提示了许多途径的协调调控，包括同步诱导D1蛋白循环，以及藻胆体生物合成减少和藻杆菌体降解增加之间的耦合。总体而言，基因表达谱分析为快速适应不同波长和强度的光的整合基因网络提供了新的见解。
We developed a transcript profiling methodology to elucidate expression patterns of the cyanobacterium Synechoeystis sp. strain PCC 6803 and used the technology to investigate changes in gene expression caused by irradiation with either intermediate-wavelength UV light (tN-B) or high-intensity white light. Several families of transcripts were altered by UV-B treatment, including mRNAs specifying proteins involved in light harvesting, photosynthesis, photoprotection, and the heat shock response. In addition, UV-B light induced the stringent response in Synechocystis, as indicated by the repression of ribosomal protein transcripts and other mRNAs involved in translation. High-intensity white light- and UV-B-mediated expression profiles overlapped in the down-regulation of photosynthesis genes and induction of heat shock response but differed in several other transcriptional processes including those specifying carbon dioxide uptake and fixation, the stringent response, and the induction profile of the high-light-inducible proteins. These two profile comparisons not only corroborated known physiological changes but also suggested coordinated regulation of many pathways, including synchronized induction of D1 protein recycling and a coupling between decreased phycobilisome biosynthesis and increased phycobillisome degradation. Overall, the gene expression profile analysis generated new insights into the integrated network of genes that adapts rapidly to different wavelengths and intensities of light.