Detection of peritoneal dissemination with near-infrared fluorescence laparoscopic imaging using a liposomal formulation of a synthesized indocyanine green liposomal derivative.
Detection of peritoneal dissemination with near-infrared fluorescence laparoscopic imaging using a liposomal formulation of a synthesized indocyanine green liposomal derivative.
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发表时间：
2015-03
影响因子：
2
通讯作者：
Isamu Hoshino;Tetsuro Maruyama;Hiromichi Fujito;Y. Tamura;A. Suganami;H. Hayashi;T. Toyota;Y. Akutsu-Y.
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作者：
Isamu Hoshino;Tetsuro Maruyama;Hiromichi Fujito;Y. Tamura;A. Suganami;H. Hayashi;T. Toyota;Y. Akutsu-Y.
背景/目的尽管传统的分期腹腔镜检查（SL）提高了腹膜播散的诊断准确性，但仍需要新的技术来提高SL的敏感性。我们在此描述了一种新的成像方法，该方法使用脂质体合成的吲哚菁绿色（ICG）脂质体衍生物LP-ICG-C18，采用近红外（NIR）荧光成像。方法和结果LP-ICG-C18是一种近红外光活化探针，其中ICG荧光团与磷脂部分共价缀合。向裸小鼠腹腔内注射胃癌细胞。12天后，以0.15 mg/kg的剂量对小鼠静脉注射LP-ICG-C18。近红外成像系统用于识别播散性肿瘤。小鼠播散性结节的检测没有任何困难。从注射或不注射脂质体制剂的小鼠中收集播散的肿瘤结节，并转移到猪腹膜腔中。近红外成像系统可清晰、迅速地确定猪腹腔内的结节。结论近红外荧光脂质体探针能有效靶向腹膜播散性肿瘤，且易于被近红外成像系统检测。这些结果保证了我们的成像系统的未来临床试验，并可能有助于胃癌患者更精确的诊断和治疗方法。
BACKGROUND/AIM Although conventional staging laparoscopy (SL) has improved the diagnostic accuracy of peritoneal dissemination, novel technology is needed to increase the sensitivity of SL. We herein describe a new imaging method employing near-infrared (NIR) fluorescence imaging using a liposomal synthesized indocyanine green (ICG) liposomal derivative, LP-ICG-C18. METHODS AND RESULTS LP-ICG-C18 is a NIR-photoactivating probe in which an ICG fluorophore is covalently conjugated with a phospholipid moiety. Nude mice were intraperitoneally injected with gastric cancer cells. Twelve days later, the mice were given intravenous injections of LP-ICG-C18 at a dose of 0.15 mg/kg. A NIR imaging system was used to identify the disseminated tumors. The disseminated nodules in mice were detected without any difficulties. Disseminated tumor nodules were collected from mice with or without injections of liposomal formulation and were transferred into the swine peritoneal cavity. The nodules in the swine peritoneal cavity were clearly and promptly defined by the NIR imaging system. CONCLUSION NIR-fluorescing liposomal probes can effectively target peritoneal disseminated tumors and can be easily detected by a NIR imaging system. These results warrant future clinical trials of our imaging system and may contribute to a more precise diagnosis and therapeutic approach for gastric cancer patients.