Computational analysis of AnmK-like kinase: New insights into the cell wall metabolism of fungi
Computational analysis of AnmK-like kinase: New insights into the cell wall metabolism of fungi
复制标题
AnmK 样激酶的计算分析：真菌细胞壁代谢的新见解
DOI：
10.1016/j.jtbi.2015.05.004
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发表时间：
2015-08-21
影响因子：
2
通讯作者：
Zhang, Hongxun
中科院分区：
文献类型：
--
作者：
Dai, Jianghong;Qu, Hong;Zhang, Hongxun
1，6-脱水-N-乙酰胞壁酸激酶（AnmK）是大肠杆菌细胞壁循环的唯一标志酶。在NCBI数据库中检索了来自57种真菌的81个真菌AnmK样激酶序列，并构建了系统发育树。对斯达氏油脂酵母中一种类似AnmK的激酶--左旋葡聚糖激酶（LGK）的三维结构进行了模拟，分子对接结果表明AnmK和LGK是保守蛋白，187 Asp和212 Asp分别是酶残基。分析表明，1，6-脱水-N-乙酰葡萄糖胺（anhGlcNAc）和/或1，6-脱水-β-D-葡萄糖胺（anhGlcN）将是AnmK样激酶的合适底物。此外，在真菌中也发现了细菌细胞壁再循环的其他特征酶的对应物。两者合计，它提出了一个假定的回收anhGlcNAc/anhGlcN，这是与细胞壁的水解，存在于真菌。这种计算分析将为真菌细胞壁的代谢提供新的见解。(C)2015爱思唯尔有限公司版权所有。
1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) is the unique enzyme that marks the recycling of the cell wall of Escherichia colt. Here, 81 fungal AnmK-like kinase sequences from 57 fungal species were searched in the NCBI database and a phylogenetic tree was constructed. The three-dimensional structure of an AnmK-like kinase, levoglucosan kinase (LGK) of the yeast Lipomyces starkeyi, was modeled; molecular docking revealed that AnmK and LGK are conserved proteins, and 187Asp, 212Asp are enzymatic residues, respectively. Analysis suggests that 1,6-anhydro-N-acetylglucosamine (anhGlcNAc) and/or 1,6-anhydro-beta-D-glucosamine (anhGlcN) would be the appropriate substrates of AnmK-like kinases. Also, the counterparts of other characteristic enzymes of cell wall recycling of bacteria were found in fungi. Taken together, it is proposed that a putative recycling of anhGlcNAc/anhGlcN, which is associated with the hydrolysis of cell walls, exists in fungi. This computational analysis will provide new insights into the metabolism of fungal cell walls. (C) 2015 Elsevier Ltd. All rights reserved.