Uniformly 13C Labeled Lignin Internal Standards for Quantitative Pyrolysis−GC−MS Analysis of Grass and Wood
Uniformly 13C Labeled Lignin Internal Standards for Quantitative Pyrolysis−GC−MS Analysis of Grass and Wood
复制标题
DOI：
10.1021/acssuschemeng.9b05926
复制
发表时间：
2019-11
影响因子：
8.4
通讯作者：
Gijs van Erven;R. de Visser;P. de Waard;W. V. van Berkel;M. Kabel
中科院分区：
文献类型：
--
作者：
Gijs van Erven;R. de Visser;P. de Waard;W. V. van Berkel;M. Kabel
随着木质纤维素增值策略的不断发展，木质素分析也需要不断进步。然而，木质素定量仍然严重依赖于非特异性的，耗时和样品的重量分析和分光光度分析。在这里，我们表明，木质素的分离物均匀13 C标记的小麦秸秆，杨柳，和道格拉斯作为“理想”的内部标准热解气相色谱高分辨率质谱（py-GC-HR-MS）分析的植物生物质，允许准确和精确的定量和结构表征的木质素在草，硬木和软木。通过HSQC NMR和py-GC-HR-MS分析对13 C木质素内标物进行了全面的结构表征，并在生物质模型系统和实际植物生物质中验证了其用于木质素定量的应用。对于所有植物来源和物种，木质素含量的测定与Klason基准的相对偏差在5%以内。这些结果建立了capab...
With the ever-advancing lignocellulose valorization strategies, lignin analyses need to advance as well. However, lignin quantification still heavily relies on unspecific, time- and sample-consuming gravimetric, and spectrophotometric analyses. Here, we demonstrate that lignin isolates from uniformly 13C-labeled wheat straw, willow, and douglas fir serve as “ideal” internal standards for pyrolysis gas chromatography high-resolution mass spectrometry (py–GC–HR-MS) analyses of plant biomass, allowing the accurate and precise quantification and structural characterization of lignin in grasses, hardwoods, and softwoods. The 13C lignin internal standards were comprehensively structurally characterized by HSQC NMR and py–GC–HR-MS analyses, and their application for lignin quantification was validated in biomass model systems and in actual plant biomass. For all botanical origins and species, the lignin content was determined within 5% relative deviation of the Klason benchmark. These results establish the capab...