The Comet assay for the evaluation of genotoxic impact in aquatic environments.
The Comet assay for the evaluation of genotoxic impact in aquatic environments.
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DOI：
10.1016/j.mrrev.2008.03.001
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Lyons, B P
中科院分区：
文献类型：
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作者：
Frenzilli, G;Nigro, M;Lyons, B P
本综述考虑了彗星测定（或单细胞凝胶电泳，SCGE）评估水生环境中基因毒素的环境影响的潜力。它重点关注过去 5 年发表的各种海洋和淡水哨兵物种的体内和原位研究。大量研究报告表明，与遗传生态毒理学中常用的其他生物标志物（例如姐妹染色单体交换或微核试验）相比，彗星测定法更加敏感。由于其高灵敏度，彗星测定广泛受到实验室程序的影响，这表明鱼类和贻贝细胞都需要标准方案。然而，在文献回顾中仍然存在各种各样的个性化彗星程序，这使得已发表的结果之间的比较通常非常困难。如果负责监测水质的国家机构要常规使用彗星测定，则需要对应用于水生物种的彗星测定进行标准化和实验室间校准。
This review considers the potential of the Comet assay (or Single Cell Gel Electrophoresis, SCGE) to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies that have been carried out in various marine and freshwater sentinel species, published in the last 5 years. A large number of the studies reviewed report that the Comet assay is more sensitive when compared with other biomarkers commonly used in genetic ecotoxicology, such as sister chromatid exchanges or micronucleus test. Due to its high sensitivity, the Comet assay is widely influenced by laboratory procedures suggesting that standard protocols are required for both fish and mussel cells. However, there are still a wide variety of personalised Comet procedures evident in the literature reviewed, making comparison between published results often very difficult. Standardization and inter-laboratory calibration of the Comet assay as applied to aquatic species will be required if the Comet assay is to be used routinely by national bodies charged with monitoring water quality.