Wide-range antifungal antagonism of Paenibacillus ehimensis IB-X-b and its dependence on chitinase and beta-1,3-glucanase production.
Wide-range antifungal antagonism of Paenibacillus ehimensis IB-X-b and its dependence on chitinase and beta-1,3-glucanase production.
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爱希门类芽孢杆菌 IB-X-b 的广泛抗真菌拮抗作用及其对几丁质酶和 β-1,3-葡聚糖酶产生的依赖性。
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
P. Susi
中科院分区：
文献类型：
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作者：
G. Aktuganov;A. Melentjev;N. Galimzianova;E. Khalikova;T. Korpela;P. Susi
此前，我们分离到一株具有抗真菌活性并产生具有杀菌潜力的裂解酶的芽孢杆菌。在本研究中，我们对该细菌进行了鉴定，并进一步研究了其抗真菌特性。在液体共培养试验中，赤霉菌IB-X-b使几种病原真菌的生物量减少了45%-75%。这种抑制伴随着真菌细胞壁的降解和菌丝形态的改变。艾希木IB-X-b的残存培养基能抑制真菌的生长，表明抑制物已分泌到培养基内。在两种主要的裂解酶中，几丁质酶只被含有几丁质的底物诱导，而β-1，3-葡聚糖酶在所有碳源中都表现出稳定的水平。纯化的几丁质酶和β-1，3-葡聚糖酶均能降解浸渍菌丝体的细胞壁，而只有后者能降解完整菌丝体的细胞壁。结果表明，β-1，3-葡聚糖酶是细胞壁水解的引发剂，而几丁质酶促进了降解过程，这两种酶的抗真菌作用机制之间存在协同作用。EB拟芽孢杆菌IB-X-b对多种真菌具有显著的抗真菌活性，具有作为植物病原真菌生防剂的潜力。
Previously, we isolated a strain of Bacillus that had antifungal activity and produced lytic enzymes with fungicidal potential. In the present study, we identified the bacterium as Paenibacillus ehimensis and further explored its antifungal properties. In liquid co-cultivation assays, P. ehimensis IB-X-b decreased biomass production of several pathogenic fungi by 45%-75%. The inhibition was accompanied by degradation of fungal cell walls and alterations in hyphal morphology. Residual medium from cultures of P. ehimensis IB-X-b inhibited fungal growth, indicating the inhibitors were secreted into the medium. Of the 2 major lytic enzymes, chitinases were only induced by chitin-containing substrates, whereas beta-1,3-glucanase showed steady levels in all carbon sources. Both purified chitinase and beta-1,3-glucanase degraded cell walls of macerated fungal mycelia, whereas only the latter also degraded cell walls of intact mycelia. The results indicate synergism between the antifungal action mechanisms of these enzymes in which beta-1,3-glucanase is the initiator of the cell wall hydrolysis, whereas the degradation process is reinforced by chitinases. Paenibacillus ehimensis IB-X-b has pronounced antifungal activity with a wide range of fungi and has potential as a biological control agent against plant pathogenic fungi.