The effects of benzodiazepines on orexinergic systems in rat cerebrocortical slices
The effects of benzodiazepines on orexinergic systems in rat cerebrocortical slices
复制标题
苯二氮卓类药物对大鼠大脑皮质切片食欲素能系统的影响
DOI：
10.1213/01.ane.0000252413.62821.2e
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发表时间：
2007-02-01
影响因子：
5.7
通讯作者：
Hirota, Kazuyoshi
中科院分区：
文献类型：
--
作者：
He, Ying;Kudo, Mihoko;Hirota, Kazuyoshi
背景技术：由于食欲素能（Orexinergic）神经元已被报道介导情绪变化，苯二氮卓类药物可能与Orexinergic neurons.METHODS：我们检测了Orexin-A（100 nM）和K+（25 mM）诱发的大鼠大脑皮层去甲肾上腺素释放中Orexin-A（100 nM）和苯二氮卓类药物受体之间的相互作用。咪达唑仑、地西泮和氟硝西泮浓度依赖性地抑制OX-A和K+诱发的去甲肾上腺素释放。咪达唑仑对orexin-A的IC_（50）（0.87 μ M，P < 0.01）显著低于地西泮和氟硝西泮（60 μ M左右），而对K ~+的IC_（50）在苯二氮类药物中无差异。结论：中枢苯二氮类受体与O能神经元之间可能无相互作用。
BACKGROUND: As orexinergic (OXergic) neurons have been reported to mediate emotional changes, benzodiazepines might interact with OXergic neurons.METHODS: We examined the interactions between OXergic neurons and benzodiazepine receptors in orexin-A (100 nM) and K+ (25 mM)-evoked norepinephrine release from rat cerebrocortical slices.RESULTS: Midazolam, diazepam, and flunitrazepam concentration-dependently inhibited both OX-A- and K+-evoked norepinephrine release. The IC50 of midazolam for orexin-A-evoked release (0.87 mu M, P < 0.01), which was insensitive to flumazenil, was significantly lower than that of diazepam and flunitrazepam (around 60 mu M), whereas the IC(50)s for K+-evoked release were not different among the benzodiazepines.CONCLUSION: There may be no interaction between OXergic neurons and central benzodiazepine receptors.