Regulation of intracellular pH in single rat zona glomerulosa cells.
Regulation of intracellular pH in single rat zona glomerulosa cells.
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单只大鼠肾小球带细胞内 pH 值的调节。
DOI：
10.1152/ajpcell.1992.262.1.c182
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Quinn,SJ
中科院分区：
文献类型：
--
作者：
Shepherd,RM;Williams,GH;Quinn,SJ
The cytosolic pH (pHi) regulation of rat adrenal zona glomerulosa (ZG) cells was studied using single-cell spectrofluorimetry. Basal pHi was similar for cells incubated in the absence or presence of the HCO3(-)-CO2 buffering system. In the absence of HCO3-, inhibition of the Na(+)-H+ exchanger by dimethylamiloride (DMA) or removal of extracellular Na+ produced substantial acidification of basal pHi. In the presence of HCO3-, neither maneuver affected basal pHi. However, removing extracellular Cl- produced a prompt alkalinization not observed in the absence of HCO3-. Alkalinizing mechanisms were examined by monitoring pHi recovery from an acid load imposed by the NH4Cl pulse technique. In the absence of HCO3-, Na+ removal or DMA addition blocked greater than 80% of pHi recovery. In the presence of HCO3-, 34% of the pHi recovery rate was inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), 34% by DMA, and 72% by Na+ removal plus DIDS. Therefore both Na(+)-H+ and anion exchangers are active in these cells, working independently to maintain basal pHi. In the absence of HCO3-, Na(+)-H+ exchange is principally responsible for pHi recovery from an acid load. In HCO(3-)-containing medium, such recovery is shared by Na(+)-H+ and Cl(-)-HCO3- exchange.
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DOI：
10.1677/joe.0.1100293
发表时间：
1986
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Radke,KJ;TaylorJr,RE;Schneider,EG
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Schneider,EG
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DOI：
10.1098/rspb.1974.0025
发表时间：
1974
期刊：
Proceedings of the Royal Society of London. Series B. Biological Sciences
影响因子：
--
作者：
J. Tait;S. Tait;R. Gould;M. Mee
通讯作者：
M. Mee
影响因子：
4.8
作者：
S. Quinn;M. Cornwall;G. Williams
通讯作者：
S. Quinn;M. Cornwall;G. Williams
Vero 细胞中 Na+/H+ 和 Cl−/HCO3− 反向转运的调节
影响因子：
5.6
作者：
T. Tønnessen;A. T. Aas;J. Ludt;H. Blomhoff;S. Olsnes
通讯作者：
S. Olsnes
血管紧张素 II 刺激的单个肾上腺肾小球细胞中的钙振荡。
DOI：
10.1073/pnas.85.15.5754
发表时间：
1988
影响因子：
11.1
作者：
Quinn,SJ;Williams,GH;Tillotson,DL
通讯作者：
Tillotson,DL