Postharvest biological control of Rhizopus rot and the mechanisms involved in induced disease resistance of peaches by Pichia membranefaciens
Postharvest biological control of Rhizopus rot and the mechanisms involved in induced disease resistance of peaches by Pichia membranefaciens
复制标题
采后根霉腐烂的生物防治及膜毕赤酵母诱导桃子抗病的机制
DOI：
10.1016/j.postharvbio.2020.111146
复制
发表时间：
2020-05-01
影响因子：
7
通讯作者：
Zhang, Hongyin
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoyun;Wu, Feng;Zhang, Hongyin
利用拮抗酵母菌对水果采后病害进行生物防治，是减少水果采后损失的有效途径。研究了膜质毕赤酵母对桃根霉腐烂病的生防效果。通过对桃抗病相关酶活性的检测和转录组分析，探讨诱导桃抗病性的机制。结果表明，生膜疫霉对桃根霉腐烂病具有明显的生防效果。该酵母菌对防御相关酶PPO、POD、PAL和CAT的活性具有潜在的诱导作用。转录组分析结果表明，膜疫霉启动了MAPK级联信号通路以及乙烯（ET）、茉莉酸（JA）和水杨酸（SA）等信号转导通路，调控桃中的转录因子（TF）。然后，这些TF进一步介导包括PR基因在内的下游防御相关基因的表达（PR 1、CHI 4和主要过敏原Pru ar 1）和谷胱甘肽S-转移酶（GST）基因（MKP 11.22和MKP 10370），这些基因被分类为植物-病原体相互作用途径（CML 48、MUK11.19和ROBHA）和参与次生代谢产物合成的基因（GGPS、PKS 5、CHS 1、CYP 75 B2、DFR、LDOX、PAL、PNC 1和ROMT）来增强桃的抗病潜力。此外，还可以促进黄酮类化合物和木质素等抗真菌物质的积累，从而提高桃的抗真菌能力。因此，本研究有助于了解拮抗酵母诱导桃抗病性的机制，并为提高桃对病原菌的防御反应提供新的病害防治策略。
Biological control of postharvest diseases of fruit by antagonistic yeast has been considered to be an effective and promising strategy to reduce the postharvest loss of fruits. In this study, the biocontrol efficacy of Pichia membrane-faciens against Rhizopus rot of peaches was investigated. Also, the study was aimed to explore the mechanisms involved in the induced disease resistance of peaches by investigating the activities of defense-related enzymes and transcriptome analysis. The results indicated that P. membranefaciens had significant biocontrol efficacy against Rhizopus rot of peaches. The activities of defense-related enzymes including PPO, POD, PAL and CAT were potentially induced by this yeast. The transcriptome analysis revealed that MAPK cascade signaling pathway and signal transduction pathways of ethylene (ET), jasmonate (JA) and salicylic acid (SA) were triggered in peaches by P. membranefaciens to regulate the transcription factors (TFs). Then, these TFs further mediated the expression of downstream defense-related genes including PR genes (PR1, CHI4 and major allergen Pru ar 1) and glutathione S-transferase (GST) genes (MKP11.22 and Atlg10370), the genes classified into plant-pathogen interaction pathways (CML48, MUK11.19 and ROBHA) and the genes involved in the synthesis of secondary metabolites (GGPS, PKS5, CHS1, CYP75B2, DFR, LDOX, PAL, PNC1 and ROMT) to enhance the disease resistance potential of peaches. Besides, the accumulation of some antifungal compounds including flavonoids and lignin was stimulated to enhance the antifungal ability of peaches. Consequently, the present study contributes to understand the mechanisms behind the induced disease resistance of peaches by antagonistic yeast, and would provide new disease control strategy by improving the defense responses of fruit against pathogens.