CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.
CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.
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用于检测核酸的 CdSe/ZnS 核壳量子点 FRET 二元寡核苷酸探针。
DOI：
10.1039/c1pp05132f
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Ju,Jingyue
中科院分区：
文献类型：
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作者：
Peng,Yiru;Qiu,Chunmei;Jockusch,Steffen;Scott,AmyM;Li,Zengmin;Turro,NicholasJ;Ju,Jingyue
我们报告的设计，合成，并表征的二元寡核苷酸探针的选择性DNA或RNA检测。该探针是基于荧光共振能量转移（FRET）从量子点（CdSe/ZnS核壳）DNA共轭物到有机染料（花青-5）DNA共轭物。供体/受体DNA缀合物与靶DNA的选择性杂交增强FRET，并观察到荧光特征的变化。
We report the design, synthesis, and characterization of a binary oligonucleotide probe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.