Use of proteomic methodology for the characterization of human milk fat globular membrane proteins
Use of proteomic methodology for the characterization of human milk fat globular membrane proteins
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DOI：
10.1006/abio.2001.5498
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发表时间：
2002-02-15
影响因子：
2.9
通讯作者：
Camilleri, P
中科院分区：
文献类型：
--
作者：
Charlwood, J;Hanrahan, S;Camilleri, P
采用蛋白质组学技术，包括二维聚丙烯酰胺凝胶电泳，然后通过原位PNGase F和胰蛋白酶消化，进行了主要的人乳脂肪球膜蛋白的表征。基质辅助激光解吸/电离四极杆飞行时间和电喷雾电离质谱鉴定了7种主要蛋白质组分：α-乳白蛋白、溶菌酶前体、β-酪蛋白、丛生蛋白、乳转铁蛋白、多聚免疫球蛋白受体前体和人乳脂肪球EGF-因子8蛋白。通过基质辅助激光解吸电离四极杆飞行时间混合质谱法测定蛋白质相关聚糖的序列信息。该聚糖分析揭示了有趣的岩藻糖基化分支模式，其可能对新生儿的母体保护免受细菌和病毒病原体攻击有影响。(C)2002 Elsevier Science（美国）。
Characterization of the major human milk fat globular membrane proteins was carried out using proteomic techniques comprising two-dimensional polyacrylamide gel electrophoresis, followed by in situ PNGase F and trypsin digestion. Matrix-assisted laser desorption/ionization quadropole time-of-flight and electrospray ionization mass spectrometry identified seven major protein components: alpha-lactalbumin, lysozyme precursor, beta-casein, clusterin, lactotransferrin, polymeric immunoglobulin receptor precursor, and human milk fat globule EGF-factor 8 protein. Sequence information on the protein-associated glycans was determined by matrix-assisted laser desorption-ionization quadropole time-of-flight hybrid mass spectrometry. This glycan analysis revealed interesting fucosylation branching patterns which may be influential in maternal protection of the newborn against bacterial and viral pathogenic attack. (C) 2002 Elsevier Science (USA).