Epigenomic Regulatory Mechanism in Vegetative Phase Transition of Malus hupehensis
Epigenomic Regulatory Mechanism in Vegetative Phase Transition of Malus hupehensis
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湖北海棠营养相变的表观基因组调控机制
DOI：
10.1021/acs.jafc.0c00478
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发表时间：
2020
影响因子：
6.1
通讯作者：
Zhao Juan
中科院分区：
文献类型：
--
作者：
Xing Libo;Qi Siyan;Zhou Hua;Zhang Wei;Zhang Chenguang;Ma Wenchun;Zhang Qingwei;Shah Kamran;Han Mingyu;Zhao Juan
在木本植物中，相变实质上影响生长和发育。虽然已经有相当大的兴趣在相位变化的监管机制，相关的表观遗传修饰仍然相对不确定。本研究以湖北海棠（Malus hupehensisleaves）为材料，研究了其成株和幼龄株的DNA甲基化变化及其转录响应.结果表明，在CG环境下，成叶和幼叶的DNA甲基化率分别为66.61%和68.3%，在CHG环境下分别为49.12%和52.44%，在CHH环境下分别为7.02%和8.22%。差异甲基化区域的数量在所有情况下分布在基因区域变化。此外，抑制成年叶片中的DNA甲基化激活吲哚-3-乙酸相关基因的转录在信号，响应和运输途径。此外，在成叶和幼叶之间，观察到SPL 2和AP 2家族基因的相反甲基化和表达模式。这两个基因家族都对M.湖北植物相转变此外，转录因子基因的基因体或启动子的高/低甲基化可导致基因表达上调/下调。WRKY（22）、NAC（21）、ERF（8）、WOX（2）、KNAT（6）、EIN 3（2）、SCL（7）、ZAT（7）和HSF（4）基因的甲基化水平在成叶中高于幼叶，而TCP（2）、MADS-box（11）和DOF（3）基因的甲基化水平则相反。甲基化和转录之间的相关性分析表明，在所有情况下，基因体的甲基化以及CG和CHG情况下启动子的甲基化与基因表达呈负相关。然而，CHH背景下启动子的甲基化与基因表达正相关。这些发现反映了基因表达表观遗传调控的多样性，可能有助于阐明M.湖北植物相转变
In woody plants, phase transitions substantially affect growth and development. Although there has been considerable interest in the regulatory mechanisms underlying phase changes, the associated epigenetic modifications remain relatively uncharacterized. We examined the DNA methylation changes and the transcriptional responses in adult and juvenileMalus hupehensisleaves. The DNA methylations were 66.61% and 68.3% in the CG context, 49.12% and 52.44% in the CHG context, and 7.02% and 8.22% in the CHH context for the adult and juvenile leaves, respectively. The number of differentially methylated regions in all contexts distributed in the genic regions varied. Additionally, inhibited DNA methylation in adult leaves activated the transcription of indole-3-acetic acid related genes in the signaling, response, and transport pathways. Moreover, the opposite methylation and expression patterns were observed for theSPLandAP2family genes between the adult and juvenile leaves. Both gene families contribute to theM. hupehensisvegetative phase transition. Furthermore, the hyper-/hypomethylation of the gene body or promoter of transcription factor genes may lead to up-/downregulated gene expression. The methylation levels of theWRKY(22),NAC(21),ERF(8),WOX(2),KNAT(6),EIN3(2),SCL(7),ZAT(7), andHSF(4) genes were higher in the adult leaves than in the juvenile leaves, whereas the opposite pattern was observed for theTCP(2),MADS-box(11), andDOF(3) genes. An analysis of the correlation between methylation and transcription indicated the methylation of the gene body in all contexts and the methylation of the promoter in the CG and CHG contexts are negatively correlated with gene expression. However, the methylation of the promoter in the CHH context is positively correlated with gene expression. These findings reflect the diversity in the epigenetic regulation of gene expression and may be useful for elucidating the epigenetic regulatory mechanism underlying theM. hupehensisvegetative phase transition.