Intercalation behavior and tensile strength of DNA-lipid films for the dental application
Intercalation behavior and tensile strength of DNA-lipid films for the dental application
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DOI：
10.1016/j.biomaterials.2004.01.006
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发表时间：
2004-11-01
期刊：
影响因子：
14
通讯作者：
Okahata, Y
中科院分区：
文献类型：
--
作者：
Fukushima, T;Hayakawa, T;Okahata, Y
在这项研究中，我们制备了DNA-脂膜，并检测了它们的插层行为和抗张强度，以此作为作为牙科材料用途的指标。在对甲苯磺酸存在下，甘氨酸、L-丙氨酸或L-谷氨酸与正烷基醇反应生成脂质。从氯仿/乙醇溶液中浇铸DNA-脂质复合体，制备了自立的、不溶于水的DNA-脂膜。DNA-脂膜与溴化乙锭形成插层络合物。这表明DNA-脂膜保持了双螺旋结构。DNA膜的拉伸强度为0.8-2.4兆帕，与用于牙科引导组织再生的商业材料(膜)兼容。我们得出结论，DNA-脂膜有可能被用作植入物表面处理的材料或作为牙科应用的骨引导支架。(C)2004爱思唯尔有限公司。保留所有权利。
In this study, we prepared DNA-lipid films and examined their intercalation behavior and tensile strength as an indicator for usefulness as a dental material. The lipids were synthesized from the reaction of glycine, L-alanine, or L-glutamic acid with n-alkyl alcohol in the presence of p-toluenesulfonic acid. The self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complex from chloroform/ethanol solution. The DNA-lipid films formed intercalation complexes with ethidium bromide. This indicates that DNA-lipid films maintain a double helical structure. The tensile strengths of DNA films were 0.8-2.4 MPa and were compatible with a commercially available material (Membrane) for guided tissue regeneration in dental use. We conclude that DNA-lipid films have potential for use as a material for the surface treatment of implanted materials or as a bone-guiding scaffold for dental application. (C) 2004 Elsevier Ltd. All rights reserved.