Control of Salmonella Enteritidis and Salmonella Gallinarum in birds by using live vaccine candidate containing attenuated Salmonella Gallinarum mutant strain
Control of Salmonella Enteritidis and Salmonella Gallinarum in birds by using live vaccine candidate containing attenuated Salmonella Gallinarum mutant strain
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DOI：
10.1016/j.vaccine.2010.01.058
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发表时间：
2010-04-01
期刊：
影响因子：
5.5
通讯作者：
Berchieri Junior, Angelo
中科院分区：
文献类型：
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作者：
Casarin Penha Filho, Rafael Antonio;de Paiva, Jacqueline Boldrin;Berchieri Junior, Angelo
理想的理想活疫苗控制沙门氏菌在商业鸡群应产生保护对各种菌株。本研究的目的是确认参与钴胺素生物合成的基因cobS和cbiA缺失的鸡沙门氏菌（SG）突变株的减毒。此外，评估其作为沙门氏菌活疫苗的用途。为了评价疫苗效力，分别进行了两个实验。来自商业棕色系鸡的禽类用于用SG野生型菌株进行攻击，来自商业白色系鸡的禽类用于用沙门氏菌（SE）野生型菌株进行攻击。在两个实验中，将鸟分成三组（A、B和C）。按照以下程序用SG突变体口服接种禽类：A组，在5日龄时接种一剂; B组，在5日龄时接种一剂，在25日龄时接种第二剂; C组，禽类保持未接种作为对照。在45日龄时，用SG野生型（棕色系）或SE野生型（白色系）经口攻击来自所有组（包括对照组）的鸟。最后，进行另一个实验以评估SG突变菌株在1日龄肉仔鸡上防止SE野生型盲肠定殖的用途。在褐鸡中评估SG野生型菌株的死亡率和全身感染;在白色鸡中评估SE野生型的粪便脱落和全身感染，并在肉鸡中评估盲肠定殖。无论是接种一剂或两剂SG突变体，都能保护褐鸡免受SG野生型的攻击。在用白色鸡的实验中，仅用两个剂量的SG突变体疫苗接种保护了鸡抵抗SE野生型的攻击。虽然SG突变体不能阻止攻毒株SE野生型在1日龄肉仔鸡盲肠中的定殖。总体而言，结果表明，SG突变体是一种有前途的沙门氏菌活疫苗候选物，其表现出良好的效力来控制对家禽业具有重要意义的两种血清型的感染。(c)2010爱思唯尔有限公司保留所有权利。
The ideal ideal live vaccine to control Salmonella in commercial chicken flocks should engender protection against various strains. The purpose of the present study was to confirm the attenuation of a Salmonella Gallinarum (SG) mutant strain with deletion on genes cobS and cbiA, that are involved in the biosynthesis of cobalamin. Furthermore, evaluate its use as a live vaccine against Salmonella. For the evaluation of the vaccine efficacy, two experiments were conducted separately. Birds from a commercial brown line of chickens were used to perform challenge with SG wild type strain and birds from a commercial white line of chickens were used to perform challenge with Salmonella Enteritidis (SE) wild type strain. In both experiments, the birds were separated in three groups (A, B and C). Birds were orally vaccinated with the SG mutant as the following programme: group A, one dose at 5 days of age; group B, one dose at 5 days of age and a second dose at 25 days of age; and group C, birds were kept unvaccinated as controls. At 45 days of age, birds from all groups, including the control, were challenged orally by SG wild type (brown line) or SE wild type (white line). Lastly, another experiment was performed to evaluate the use of the SG mutant strain to prevent caecal colonization by SE wild type on 1-day-old broiler chicks. Mortality and systemic infection by SG wild type strain were assessed in brown chickens; faecal shedding and systemic infection by SE wild type were assessed in white chickens and caecal colonization was assessed in broiler chicks. Either vaccination with one or two doses of SG mutant, were capable to protect brown chickens against SG wild type. In the experiment with white chickens, only vaccination with two doses of SG mutant protected the birds against challenge with SE wild type. Although, SG mutant could not prevent caecal colonization in 1-day-old broiler chicks by the challenge strain SE wild type. Overall, the results indicated that SG mutant is a promising Salmonella live vaccine candidate that demonstrated good efficacy to control the infection by two serotypes of major importance to the poultry industry. (c) 2010 Elsevier Ltd. All rights reserved.