HUMAN CDNA CLONES FOR 4 SPECIES OF G-ALPHA-S SIGNAL TRANSDUCTION PROTEIN
HUMAN CDNA CLONES FOR 4 SPECIES OF G-ALPHA-S SIGNAL TRANSDUCTION PROTEIN
复制标题
DOI：
10.1073/pnas.83.23.8893
复制
发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
NIRENBERG, M
中科院分区：
文献类型：
--
作者：
BRAY, P;CARTER, A;NIRENBERG, M
用寡核苷酸探针筛选源自人脑的.lambda.gt11 cDNA文库，寻找编码.α的重组体。 G信号转导蛋白的亚基。用两种探针检测到十一个αs克隆并进行表征。克隆了四种类型的αs cDNA，其在对应于氨基酸残基71-88的区域中的核苷酸序列不同。这些克隆的不同之处在于α2氨基酸残基71（谷氨酸或天冬氨酸）的密码子、接下来的15个氨基酸残基的密码子的存在或不存在、以及相邻的丝氨酸残基的存在或不存在。 S1核酸酶保护实验揭示了至少两种形式的αs mRNA。提出了通过前体RNA的选择性剪接产生四种αs mRNA的机制。
.lambda.gt11 cDNA libraries derived from human brain were screened with oligonucleotide probes for recombinants that code for .alpha. subunits of G signal transduction proteins. Eleven .alpha.s clones were detected with both probes and characterized. Four types of .alpha.s cDNA were cloned that differ in nucleotide sequence in the region that corresponds to amino acid residues 71-88. The clones differ in the codon for .alpha.2 amino acid residue 71 (glutamic acid or aspartic acid), the presence or absence of codons for the next 15 amino acid residues, and the presence or absence of an adjacent serine residue. S1 nuclease protection experiments revealed at least two forms of .alpha.s mRNA. A mechanism for generating four species of .alpha.s mRNA by alternative splicing of precursor RNA is proposed.