Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover
Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover
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DOI：
10.1016/j.biortech.2011.04.005
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发表时间：
2011-07-01
影响因子：
11.4
通讯作者：
Singh, Seema
中科院分区：
文献类型：
--
作者：
Li, Chenlin;Cheng, Gang;Singh, Seema
研究了离子液体（IL）和氨纤维膨胀（AFEX）预处理，以开发第一个直接并排比较评估各自对生物质结构，组成，过程质量平衡和酶糖化效率的影响。AFEX预处理完全保留植物碳水化合物，而IL预处理提取76%的半纤维素。与AFEX相反，从IL预处理回收的玉米秸秆的天然晶体结构被显著破坏。对于这两种技术，超过70%的理论糖产量后，使用商业酶鸡尾酒水解48小时达到。IL预处理需要较少的酶加载和较短的水解时间，以达到90%的产率。半纤维素酶的添加导致AFEX预处理的玉米秸秆的葡萄糖和木糖的产率的显着改善，但不是IL预处理的秸秆。这些结果为IL和AFEX预处理的机制以及各自的优缺点提供了新的见解。爱思唯尔有限公司出版
Ionic liquid (IL) and ammonia fiber expansion (AFEX) pretreatments were studied to develop the first direct side-by-side comparative assessment on their respective impacts on biomass structure, composition, process mass balance, and enzymatic saccharification efficiency. AFEX pretreatment completely preserves plant carbohydrates, whereas IL pretreatment extracts 76% of hemicellulose. In contrast to AFEX, the native crystal structure of the recovered corn stover from IL pretreatment was significantly disrupted. For both techniques, more than 70% of the theoretical sugar yield was attained after 48 h of hydrolysis using commercial enzyme cocktails. IL pretreatment requires less enzyme loading and a shorter hydrolysis time to reach 90% yields. Hemicellulase addition led to significant improvements in the yields of glucose and xylose for AFEX pretreated corn stover, but not for IL pretreated stover. These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each. Published by Elsevier Ltd.