Biomimetic fabrication of fibrin/apatite composite material.
Biomimetic fabrication of fibrin/apatite composite material.
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DOI：
10.1002/jbm.a.31777
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
Rui Yoh;T. Matsumoto;J. Sasaki;T. Sohmura
中科院分区：
文献类型：
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作者：
Rui Yoh;T. Matsumoto;J. Sasaki;T. Sohmura
在不同的pH条件和氟浓度下，将Ca和PO4溶液双向扩散到纤维蛋白凝胶中，生成模拟生物矿化的有机/无机复合材料。该体系生成的矿物结晶度高于溶液矿化体系生成的矿物。纤维蛋白凝胶中生成的矿物质随pH值的变化而变化：非控制pH条件下的二水磷酸二钙（DCPD）， pH值为7.4时的DCPD与磷酸八钙（OCP）混合物，pH值为9.0时的OCP与羟基磷灰石（HAp）混合物。当氟离子在2 ~ 500ppm范围内加入时，在pH 7.4下产生的矿物由OCP/HAp转变为HAp/氟磷灰石（FAp）。此外，所得矿物的结晶度随氟离子浓度的增加而增加，溶解度与结晶度呈负相关。综上所述，我们建立了一种新的合成纤维蛋白和磷酸钙有机/无机复合材料的制备方法，并揭示了合成材料中矿物质的特性可以通过制备条件进行控制。
Bidirectional diffusion of the Ca and PO4 solutions into the fibrin gel was performed at various pH conditions and fluoride concentrations to generate organic/inorganic composite materials mimicking biomineralization. The minerals produced in this system had a higher crystallinity than those generated by the solution mineralization system. The minerals generated in fibrin gel varied depending on the pH conditions as follows: Dicalcium phosphate dihydrate (DCPD) in the noncontrolled pH solution, the DCPD and octacalcium phosphate (OCP) mixture at pH 7.4, and the OCP and hydroxyapatite (HAp) mixture at pH 9.0. When fluoride ions were added in the range of 2-500 ppm, the minerals produced at pH 7.4 altered from OCP/HAp to HAp/fluorapatite (FAp). In addition, the crystallinity of the obtained minerals increased with an increase in fluoride ion concentration, and the solubility was inversely correlated to crystallinity. In conclusion, we established a novel fabrication method for synthesizing organic/inorganic composite materials composed of fibrin and calcium phosphate and revealed that the characteristics of the minerals in the synthesized material can be controlled by the fabrication condition.