Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry
Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry
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DOI：
10.1194/jlr.d003715
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发表时间：
2010-06-01
影响因子：
6.5
通讯作者：
Brown, H. Alex
中科院分区：
文献类型：
--
作者：
Ivanova, Pavlina T.;Milne, Stephen B.;Brown, H. Alex
巨噬细胞中甘油磷脂种类的大规模分析和分析促进了几种罕见和非典型甘油磷脂种类的鉴定。通过使用液相色谱串联质谱法并将稀有脂质的洗脱和碎片化特性与合成标准品进行比较，我们能够鉴定一系列醚连接的磷脂酰肌醇（PI）、磷脂酸、磷脂酰丝氨酸（PS）、极长链磷脂酰乙醇胺（PE），和磷脂酰胆碱（PC）以及磷脂酰苏氨酸（PT）和巨噬细胞中广泛收集的含奇数碳脂肪酸的磷脂。对来自不同巨噬细胞的甘油磷脂进行了全面的定性分析。在巨噬细胞样RAW 264.7细胞的磷脂分析过程中，我们鉴定了数十种罕见或以前未表征的磷脂，包括醚连接的PI、PS和甘油磷脂酸、PT以及含有非常长的多不饱和脂肪酸的PC和PT。此外，大量的磷脂含有至少一个奇数碳脂肪酸被确定。使用相同的方法，我们还在常驻腹膜巨噬细胞、泡沫细胞和小鼠骨髓源性巨噬细胞中鉴定了许多相同种类的甘油磷脂。伊万诺娃，P.T.，S. B。Milne和H. A.布朗巨噬细胞中非典型醚连接甘油磷脂的质谱鉴定。J. Lipid Res. 2010. 51：1581-1590。
A large scale profiling and analysis of glycerophospholipid species in macrophages has facilitated the identification of several rare and atypical glycerophospholipid species. By using liquid chromatography tandem mass spectrometry and comparison of the elution and fragmentation properties of the rare lipids to synthetic standards, we were able to identify an array of ether-linked phosphatidylinositols (PIs), phosphatidic acids, phosphatidylserines (PSs), very long chain phosphatidylethanolamines (PEs), and phosphatidylcholines (PCs) as well as phosphatidylthreonines (PTs) and a wide collection of odd carbon fatty acid-containing phospholipids in macrophages. A comprehensive qualitative analysis of glycerophospholipids from different macrophage cells was conducted. During the phospholipid profiling of the macrophage-like RAW 264.7 cells, we identified dozens of rare or previously uncharacterized phospholipids, including ether-linked PIs, PSs, and glycerophosphatidic acids, PTs, and PCs and PTs containing very long polyunsaturated fatty acids. Additionally, large numbers of phospholipids containing at least one odd carbon fatty acid were identified. Using the same methodology, we also identified many of the same species of glycerophospholipids in resident peritoneal macrophages, foam cells, and murine bone marrow derived macrophages.-Ivanova, P. T., S. B. Milne, and H. A. Brown. Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry. J. Lipid Res. 2010. 51: 1581-1590.