Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice.
Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice.
复制标题
蓝绿藻（发菜公社）的降血脂作用归因于其非脂质部分通过减少 C57BL/6J 小鼠肠道胆固醇吸收。
DOI：
10.1089/jmf.2014.0121
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发表时间：
2015
影响因子：
2.4
通讯作者：
Lee,Ji-Young
中科院分区：
文献类型：
--
作者：
Ku,ChaiSiah;Kim,Bohkyung;Pham,ThoX;Yang,Yue;Weller,CurtisL;Carr,TimothyP;Park,Young-Ki;Lee,Ji-Young
我们之前证明发菜（NO）是一种蓝绿藻（BGA），在体内发挥降血脂作用，其脂质提取物在体外调节参与胆固醇和脂质代谢的基因的表达。本研究的目的是探讨与钝顶螺旋藻（SP）相比，NO的降血脂作用是否归因于体内藻类脂质或脱脂组分。雄性 C57BL/6J 小鼠饲喂含有 2.5% 或 5% BGA（w/w）或相当于 5% BGA 的脂质提取物的 AIN-93M 饮食 4 周，以测量血浆和肝脏脂质、肝脏基因表达、肠道胆固醇吸收和粪便甾醇排泄。与对照组相比，2.5% 和 5% NO 喂养组的血浆总胆固醇 (TC) 显着降低，而 5% NO 组的血浆甘油三酯 (TG) 水平也降低。然而，NO 有机提取物 (NOE) 和 SP 喂养组均未改变血浆脂质。 5% NO 喂养的小鼠中，甾醇调节元件结合蛋白 2、3-羟基-3-甲基-戊二酰辅酶 A 还原酶 (HMGR)、肉碱棕榈酰转移酶-1α 和酰基辅酶 A 氧化酶 1 的肝脏 mRNA 水平被诱导，而各组之间的肝脏脂肪生成基因表达没有显着变化。 NO显着降低肠道胆固醇吸收，但NOE和SP组则不然。当 HepG2 细胞和原代小鼠肝细胞与 NOE 和 SP 有机提取物 (SPE) 一起孵育时，HMGR、低密度脂蛋白受体和脂肪酸合酶的蛋白水平显着降低。总之，NO 的非脂质部分主要通过抑制肠道胆固醇吸收和增加肝脏脂肪酸氧化来发挥降低 TC 和 TG 的作用。
We previously demonstrated thatNostoc communevar.sphaeroidsKützing (NO), a blue-green alga (BGA), exerts a hypolipidemic effectin vivoand its lipid extract regulates the expression of genes involved in cholesterol and lipid metabolismin vitro. The objective of this study was to investigate whether the hypolipidemic effect of NO is attributed to an algal lipid or a delipidated fractionin vivocompared withSpirulina platensis(SP). Male C57BL/6J mice were fed an AIN-93M diet containing 2.5% or 5% of BGA (w/w) or a lipid extract equivalent to 5% of BGA for 4 weeks to measure plasma and liver lipids, hepatic gene expression, intestinal cholesterol absorption, and fecal sterol excretion. Plasma total cholesterol (TC) was significantly lower in 2.5% and 5% NO-fed groups, while plasma triglyceride (TG) levels were decreased in the 5% NO group compared with controls. However, neither NO organic extract (NOE) nor SP-fed groups altered plasma lipids. Hepatic mRNA levels of sterol regulatory element-binding protein 2, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), carnitine palmitoyltransferase-1α, and acyl-CoA oxidase 1 were induced in 5% NO-fed mice, while there were no significant changes in hepatic lipogenic gene expression between groups. NO, but not NOE and SP groups, significantly decreased intestinal cholesterol absorption. When HepG2 cells and primary mouse hepatocytes were incubated with NOE and SP organic extract (SPE), there were marked decreases in protein levels of HMGR, low-density lipoprotein receptor, and fatty acid synthase. In conclusion, the nonlipid fraction of NO exerts TC and TG-lowering effects primarily by inhibiting intestinal cholesterol absorption and by increasing hepatic fatty acid oxidation, respectively.