Target recycling amplification for label-free and sensitive colorimetric detection of adenosine triphosphate based on un-modified aptamers and DNAzymes
Target recycling amplification for label-free and sensitive colorimetric detection of adenosine triphosphate based on un-modified aptamers and DNAzymes
复制标题
基于未修饰适体和脱氧核糖核酸酶的三磷酸腺苷无标记和灵敏比色检测的目标回收扩增
DOI：
10.1016/j.aca.2014.04.028
复制
发表时间：
2014-05-30
影响因子：
6.2
通讯作者：
Chai, Yaqin
中科院分区：
文献类型：
--
作者：
Gong, Xue;Li, Jinfu;Chai, Yaqin
基于目标循环扩增，利用未修饰的适体和DNAzymes建立了一种新的无标记、简单、灵敏的ATP比色检测方法。模型靶分子（ATP）与dsDNA探针的相应适配体的结合导致g -四重体序列的释放。结合ATP的适体可被核酸外切酶III进一步降解释放ATP， ATP可再次结合dsDNA探针的适体，启动靶循环扩增过程。由于这种靶循环扩增，g -四重体序列的释放量显著增加。随后，这些g -四重体序列结合血红素形成大量模拟过氧化物酶的DNAzymes，导致探针溶液的颜色显著增强，用于亚纳摩尔（0.33 nM）水平的高灵敏度ATP比色检测。我们的方法对ATP对其他控制分子有很高的选择性，并且可以在一个单一的均匀溶液中进行，这使得我们的传感方法对其他小分子和蛋白质的灵敏比色检测具有很大的潜力。(C) 2014 Elsevier B.V.版权所有
Based on target recycling amplification, the development of a new label-free, simple and sensitive colorimetric detection method for ATP by using un-modified aptamers and DNAzymes is described. The association of the model target molecules (ATP) with the corresponding aptamers of the dsDNA probes leads to the release of the G-quadruplex sequences. The ATP-bound aptamers can be further degraded by Exonuclease III to release ATP, which can again bind the aptamers of the dsDNA probes to initiate the target recycling amplification process. Due to this target recycling amplification, the amount of the released G-quadruplex sequences is significantly enhanced. Subsequently, these G-quadruplex sequences bind hemin to form numerous peroxidase mimicking DNAzymes, which cause substantially intensified color change of the probe solution for highly sensitive colorimetric detection of ATP down to the sub-nanomolar (0.33 nM) level. Our method is highly selective toward ATP against other control molecules and can be performed in one single homogeneous solution, which makes our sensing approach hold great potential for sensitive colorimetric detection of other small molecules and proteins. (C) 2014 Elsevier B.V. All rights reserved.