Cloning and sequencing of the bovine flavocytochrome b subunit proteins, gp91-phox and p22-phox: comparison with other known flavocytochrome b sequences.
Cloning and sequencing of the bovine flavocytochrome b subunit proteins, gp91-phox and p22-phox: comparison with other known flavocytochrome b sequences.
复制标题
牛黄素细胞色素 b 亚基蛋白 gp91-phox 和 p22-phox 的克隆和测序：与其他已知黄素细胞色素 b 序列的比较。
DOI：
10.1002/jlb.64.1.114
复制
发表时间：
1998
影响因子：
5.5
通讯作者：
Quinn,MT
中科院分区：
文献类型：
--
作者：
Davis,AR;Mascolo,PL;Bunger,PL;Sipes,KM;Quinn,MT
Neutrophils play an essential role in the cellular defense of the bovine mammary gland and compromised leukocyte function has been linked to the development of bovine mastitis. During mastitis, large numbers of leukocytes migrate into the mammary tissues where they become activated, resulting in the assembly of neutrophil membrane and cytosolic proteins to form a superoxide aniongenerating complex known as the NADPH oxidase. The key membrane‐associated component of the NADPH oxidase is flavocytochromeb, which is a heterodimer ofp22‐phoxand gp91‐phox. Currently, only the human, porcine, murine, and rattusp22‐phoxand the human, porcine, and murine gp91‐phoxgene sequences are known. Because of the important role neutrophils play in bovine host defense, we carried out studies to clone, sequence, and analyze expression of bovine flavocytochromeb. Using polymerase chain reaction cloning techniques and a bovine spleen cDNA library we have cloned both of the bovine flavocytochromebsubunits, p22‐phoxand gp91‐phox. Comparison of the bovine sequences with those of other species also revealed important information regarding key structural features of gp91‐phoxandp22‐phox, including location of putative glycosylation sites. This study greatly contributes to our understanding of the potential functional sites of the flavocytochromebsubunits as well as providing information that can be used to study the role of neutrophils in bovine inflammatory diseases such as mastitis.J. Leukoc. Biol. 64: 114–123; 1998.
