Cluster analysis-based physiological classification and morphological properties of inhibitory neurons in layers 2-3 of monkey dorsolateral prefrontal cortex
Cluster analysis-based physiological classification and morphological properties of inhibitory neurons in layers 2-3 of monkey dorsolateral prefrontal cortex
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DOI：
10.1152/jn.00156.2005
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发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
Lewis, DA
中科院分区：
文献类型：
--
作者：
Krimer, LS;Zaitsev, AV;Lewis, DA
在灵长类动物中，人们对新皮层神经元的内在电生理特性及其形态学相关性知之甚少。为了对猴背外侧前额叶皮层2 - 3层的抑制细胞（中间神经元）进行分类，我们在切片制备中使用了全细胞电压记录和细胞内标记，并随后进行了形态学重建。有规律的尖峰锥体细胞也包括在样品中。利用聚类分析作为一种多变量探索技术，成功地将神经元分离成三个生理簇：规则、中间和快速尖峰细胞。当神经元的形态类型映射到生理簇上时，规则尖刺细胞簇包含所有锥体细胞，而中间和快速尖刺细胞簇仅由中间神经元组成。快速穗突细胞簇包含所有的枝形细胞和大部分局部、中等和宽乔木（篮）中间神经元。中间穗状细胞簇主要由神经胶质状或垂直定向（双束）中间神经元组成。因此，定量方法使我们能够证明猴子前额叶皮层神经元固有的电生理特性定义了不同的细胞类型，这些细胞类型也显示出不同的形态。
In primates, little is known about intrinsic electrophysiological properties of neocortical neurons and their morphological correlates. To classify inhibitory cells (interneurons) in layers 2 - 3 of monkey dorsolateral prefrontal cortex we used whole cell voltage recordings and intracellular labeling in slice preparation with subsequent morphological reconstructions. Regular spiking pyramidal cells have been also included in the sample. Neurons were successfully segregated into three physiological clusters: regular-, intermediate-, and fast-spiking cells using cluster analysis as a multivariate exploratory technique. When morphological types of neurons were mapped on the physiological clusters, the cluster of regular spiking cells contained all pyramidal cells, whereas the intermediate- and fast-spiking clusters consisted exclusively of interneurons. The cluster of fast-spiking cells contained all of the chandelier cells and the majority of local, medium, and wide arbor ( basket) interneurons. The cluster of intermediate spiking cells predominantly consisted of cells with the morphology of neurogliaform or vertically oriented (double-bouquet) interneurons. Thus a quantitative approach enabled us to demonstrate that intrinsic electrophysiological properties of neurons in the monkey prefrontal cortex define distinct cell types, which also display distinct morphologies.