Postnatal cytokines and boosts improve chimerism and hematological parameters in beta-thalassemic mice transplanted in utero.
Postnatal cytokines and boosts improve chimerism and hematological parameters in beta-thalassemic mice transplanted in utero.
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出生后细胞因子和增强剂可改善子宫内移植的β地中海贫血小鼠的嵌合状态和血液学参数。
DOI：
10.1097/00007890-200105270-00025
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发表时间：
2001
期刊：
影响因子：
6.2
通讯作者：
Carrier,E
中科院分区：
文献类型：
--
作者：
Donahue,J;Gilpin,E;Young,D;Carrier,E
我们建立了非贫血、β-地中海贫血小鼠的宫内移植模型，以研究嵌合体、耐受性以及供体细胞对细胞因子和出生后增强反应的血液学参数的变化。我们已经记录了与对照组相比，纯合子胎儿存活率提高了40%。低水平的混合嵌合体通过出生后细胞因子治疗和增强得到改善，并与血红蛋白水平、网织红细胞计数和铁储存的改善有关。细胞毒试验显示，对照组小鼠对供体细胞的反应高于子宫移植动物(效靶比50：1，移植组8.66%，对照组51.85%，P=0.0003)，表明免疫耐受。产前对同种异体细胞的耐受与出生后加强免疫相结合可能成为改善β-地中海贫血患者溶血性贫血的一种有效、无毒的策略。
Wehave developed a murine model of in utero transplantation in nonanemic, β-thalassemic mice to study chimerism, tolerance, and changes inhematological parameters in response to cytokines and postnatal boosts withdonor cells. We have documented improved survival of homozygous fetuses by 40% as compared with controls. Low-level, mixed chimerism was improved bypostnatal cytokine therapy and boosts and was associated with improvement inhemoglobin levels, reticulocyte counts, and iron stores. Cytotoxicity assaysdemonstrated higher responses to donor cells in control mice as compared within utero transplanted animals (at 50: 1 effector to target ratios, transplantedmice showed 8.66% target lysis and control mice showed 51.85% target lysis, P= 0.0003), indicatingtolerance. The combination of prenatal tolerance to allogeneic cells withpostnatal boosts in primed hosts may become an effective, nontoxic strategyfor the improvement of hemolytic anemia in β-thalassemicpatients.