Nucleotide sequence analysis of isolates of human T-lymphotropic virus type 1 of diverse geographical origins.
Nucleotide sequence analysis of isolates of human T-lymphotropic virus type 1 of diverse geographical origins.
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不同地理来源的人类 T 淋巴细胞病毒 1 型分离株的核苷酸序列分析。
DOI：
10.1089/aid.1991.7.923
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发表时间：
1991
影响因子：
1.5
通讯作者：
Trowbridge,DB
中科院分区：
文献类型：
--
作者：
Ratner,L;Philpott,T;Trowbridge,DB
测定了可能起源于加勒比海的人类嗜T淋巴细胞病毒1型（HTLV-1）分离株（HTLV-1CH）和扎伊尔分离株（HTLV-1 EL）的长末端重复序列（LTR）、gag、蛋白酶基因和pol的核苷酸序列，为这些分离株提供了完整的前病毒序列。这些序列进行了比较，从以前分析的分离株。核苷酸序列差异1.2-3.3%的分离株，其中完整的遗传信息是可用的。核苷酸序列多样性在基因组中分布相对均匀，LTR的差异为1.3-5.2%，ag的差异为1.1-2.9%，蛋白酶基因的差异为0.7-2.1%，pol的差异为0.9 - 2.5%，env的差异为0.9-2.4%，rex的差异为0.0-1.4%，tax的差异为0.1-2.6%。总体上有1.2-2.3%的氨基酸差异，0.8-1.6%的非保守氨基酸改变。在对转录活性或Tax应答重要的LTR区域中未发现核苷酸差异。在雷克斯反应元件，核苷酸的差异主要是在环，而不是茎结构，因此，保持整体二级结构所需的雷克斯活动。序列差异的进化树分析表明，不同的HTLV 1菌株根据地理来源的主要聚类。此外，在位于两个外源基因之间的负链DNA上还发现了一个开放阅读框，该开放阅读框在HTLV 1株间的核苷酸序列变异为0.1-6.9%。HTLV-1毒株之间有限的序列变异与人类免疫缺陷病毒（HIV）毒株之间广泛的异质性形成鲜明对比。
Nucleotide sequences for long terminal repeat (LTR),gag, the protease gene, andpolof a human T-lymphotropic virus type 1 (HTLV-1) isolate of probable Caribbean origin (HTLV-1CH) and a Zairian isolate (HTLV-1EL) were determined providing complete proviral sequences for these isolates. These sequences were compared with those available from previously analyzed isolates. Nucleotide sequence differences of 1.2-3.3% were identified among isolates for which complete genetic information was available. Nucleotide sequence diversity was distributed relatively evenly over the genome with 1.3-5.2% differences in the LTR, 1.1-2.9% differences ingag, 0.7-2.1% differences in the protease gene, 0.9-2.5% differences inpol, 0.9-2.4% differences inenv, 0.0-1.4% differences inrex, and 0.1-2.6% differences intax. There were 1.2-2.3% amino acid differences overall, with 0.8-1.6% nonconservative amino acid alterations. Nucleotide differences were not found in regions of the LTR which are important for transcriptional activity or Tax response. Within the Rex-response element, nucleotide differences were found predominantly in loop rather than stem structures, thus, maintaining the overall secondary structure necessary for Rex activity. Evolutionary tree analysis of the sequence differences suggests a predominant clustering of different HTLV1 strains according to geographical origin. An open reading frame was also identified on the minus DNA strand situated between theenvandrex/taxgenes which exhibits 0.1-6.9% nucleotide sequence variation among HTLV1 strains. The limited sequence variation among HTLV-1 strains is in striking contrast to the extensive heterogeneity seen among human immunodeficiency virus (HIV) strains.