Endothelin-1 inhibits adipogenesis: Role of phosphorylation of Akt and ERK1/2
Endothelin-1 inhibits adipogenesis: Role of phosphorylation of Akt and ERK1/2
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DOI：
10.1016/j.febslet.2006.09.032
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Ullrich, Axel
中科院分区：
文献类型：
--
作者：
Bhattacharya, Indranil;Ullrich, Axel
在脂肪形成中，生长因子起着至关重要的作用。采用血清耗竭的方法，研究了内皮素-1（ET-1）和表皮生长因子（EGF）单独或联合作用对3 T3-L1细胞脂肪分化的影响。ET-I刺激引起抗脂肪形成反应，并且这种作用在用EGF治疗后增强。EGF和ET-1的共同治疗阻断了脂肪形成标志物CIEBP α和PPAR γ的表达。在抑制脂肪形成之前，Akt磷酸化的双相（早期和晚期）衰减。我们认为ET-1和EGF联合治疗可诱导更有效的抗脂肪形成反应，包括Erk-1/2磷酸化增加和Akt磷酸化的双相减弱。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin-1 (ET-1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3-L1 cells. ET-I stimulation caused an anti-adipogenic response and this effect was potentiated upon treatment with EGF. Co-treatment with EGF and ET-1 blocked the expression of CIEBP alpha and PPAR gamma, the adipogenic markers. The inhibition of adipogenesis was preceded by a biphasic (early and late) attenuation of Akt phosphorylation. We suggest that treatment with ET-1 and EGF together induce a more potent anti-adipogenic response, involving increased Erk-1/2 phosphorylation and biphasic attenuation of Akt phosphorylation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.