ScRALF3, a secreted RALF-like peptide involved in cellcell communication between the sporophyte and the female gametophyte in a solanaceous species
ScRALF3, a secreted RALF-like peptide involved in cellcell communication between the sporophyte and the female gametophyte in a solanaceous species
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DOI：
10.1111/tpj.12096
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发表时间：
2013-03-01
期刊：
影响因子：
7.2
通讯作者：
Matton, Daniel P.
中科院分区：
文献类型：
--
作者：
Chevalier, Eric;Loubert-Hudon, Audrey;Matton, Daniel P.
小分子肽通过细胞间的通讯调节植物发育的各个方面.这些信号事件在生殖过程中特别重要，调节配子发育和胚胎发生。快速碱化因子（RALF）样基因是一个编码分泌肽的大基因家族，具有特异性或普遍存在的表达模式。以前，5 RALF样基因与潜在的参与生殖过程中分离出茄chacoense。在这里，我们表明ScRALF3是雌配子体发育的重要肽调节剂。它的表达是生长素诱导的，在受精前后受到严格的调控。通过RNA干扰下调ScRALF3表达导致产生较小的果实，其产生较少的种子，这是由于胚囊的不适当发育。缺陷包括胚囊核极化的丧失，以及异步分裂的增加，导致细胞功能障碍和在巨配子发生期间的过早胚囊发育停滞。原位杂交和GUS染色结果表明，ScRALF3在胚囊、珠被和珠心周围的孢子体组织中表达。如对于分泌肽所预期的，在整个分泌途径中检测到来自ScRALF3GFP融合构建体的荧光。因此，ScRALF 3分泌肽可能直接参与调节胚珠发育期间雌配子体及其周围孢子体组织之间细胞通讯的多个方面。
Small peptides have been shown to regulate numerous aspects of plant development through cellcell communication. These signaling events are particularly important during reproduction, regulating gamete development and embryogenesis. Rapid alkalinization factor (RALF)-like genes, a large gene family that encodes secreted peptides, have specific or ubiquitous expression patterns. Previously, five RALF-like genes with potential involvement during reproduction were isolated from Solanum chacoense. Here, we show that ScRALF3 is an important peptide regulator of female gametophyte development. Its expression, which is auxin-inducible, is strictly regulated before and after fertilization. Down-regulation of ScRALF3 expression by RNA interference leads to the production of smaller fruits that produce fewer seeds, due to improper development of the embryo sacs. Defects include loss of embryo sac nuclei polarization, as well as an increase in asynchronous division, accounting for cellular dysfunctions and premature embryo sac development arrest during megagametogenesis. ScRALF3 is expressed in the sporophytic tissue surrounding the embryo sac, the integument and the nucellus, as revealed by in situ hybridization and GUS staining. As expected for a secreted peptide, fluorescence from an ScRALF3GFP fusion construct is detected throughout the secretory pathway. Therefore, the ScRALF3 secreted peptide may be directly involved in the regulation of multiple aspects of cellcell communication between the female gametophyte and its surrounding sporophytic tissue during ovule development.