Exploration of Human Salivary Microbiomes--Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects.
Exploration of Human Salivary Microbiomes--Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects.
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人类唾液微生物组的探索——深入了解龋齿和无龋受试者微生物群落结构的新特征。
DOI：
10.1371/journal.pone.0147039
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zhou J;Jiang N;Wang S;Hu X;Jiao K;He X;Li Z;Wang J
近年来，高通量测序提高了人们对龋齿微生物病原学的认识，但有龋齿和无龋齿的人类口腔中微生物群落结构的特点尚不完全清楚。为了更好地了解这些特征，我们利用Illumina MiSeq高通量测序对来自中国甘肃东乡同镇的20份唾液样本（10份无龋和10份有龋）进行了分析。20名受试者唾液样本共鉴定出5113个otu （Operational Taxonomic Units， 97%截断率）。两组的比较表明：(1)优势门在两组之间是不变的；（ii）龋组细孔菌属、双歧杆菌属、硒单胞菌属、奥氏菌属、副卡多氏菌属、卡多氏菌属、黄杆菌属、Terrimonas属、伯氏菌属和孢子杆菌属的相对丰度显著高于对照组（P < 0.05）；（iii） 4个相对丰度较低的属（平均< 0.01%），包括2个龋病特征属（Chryseobacterium和Scardovia），在属和OTU水平上显著影响微生物群落结构。此外，通过共现和主成分分析，在龋病样本中检测到致病属的共流行，但在无龋样本中，聚集性属的功能更具随机性。这一结果表明，协同效应可能会影响龋齿微生物群落的聚集，而竞争可能在控制无龋齿组的微生物群落方面发挥更主要的作用。本研究对有龋组和无龋组微生物群落特征的研究，有助于提高对龋齿微生物病原学的认识，并有助于今后龋齿的预防和治疗。
Recently, high-throughput sequencing has improved the understanding of the microbiological etiology of caries, but the characteristics of the microbial community structure in the human oral cavity with and without caries are not completely clear. To better understand these characteristics, Illumina MiSeq high-throughput sequencing was utilized to analyze 20 salivary samples (10 caries-free and 10 caries) from subjects from the same town in Dongxiang, Gansu, China. A total of 5,113 OTUs (Operational Taxonomic Units, 97% cutoff) were characterized in all of the salivary samples obtained from the 20 subjects. A comparison of the two groups revealed that (i) the predominant phyla were constant between the two groups; (ii) the relative abundance of the genera Veillonella, Bifidobacterium, Selenomonas, Olsenella, Parascardovia, Scardovia, Chryseobacterium, Terrimonas, Burkholderia and Sporobacter was significantly higher in the group with caries (P < 0.05); and (iii) four genera with low relative abundance (< 0.01% on average), including two characteristic genera in caries (Chryseobacterium and Scardovia), significantly influenced the microbial community structure at the genus and OTU levels. Moreover, via co-occurrence and principal component analyses, the co-prevalence of the pathogenic genera was detected in the caries samples, but in the caries-free samples, the function of clustered genera was more random. This result suggests that a synergistic effect may be influencing the assembly of the caries microbial community, whereas competition may play a more dominant role in governing the microbial community in the caries-free group. Our findings regarding the characteristics of the microbial communities of the groups with and without caries might improve the understanding of the microbiological etiology of caries and might improve the prevention and cure of caries in the future.