Reading two bases twice: Mammalian antizyme frameshifting in yeast
Reading two bases twice: Mammalian antizyme frameshifting in yeast
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DOI：
10.1002/j.1460-2075.1996.tb00478.x
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发表时间：
1996-03-15
期刊：
影响因子：
11.4
通讯作者：
Atkins, JF
中科院分区：
文献类型：
--
作者：
Matsufuji, S;Matsufuji, T;Atkins, JF
程序化翻译移码对于哺乳动物鸟氨酸脱羧酶抗酶的表达是必不可少的，该蛋白质参与细胞内多胺的调节。发现含有抗酶移码信号的盒在酵母（Saccharomyces cerevisiae）中指导高水平（16%）移码。与哺乳动物系统中的+1移码相反，在酵母中，通过-2移码达到相同的框架。两个碱基读两遍。-2移码可能是由mRNA的滑动和与P位点中的tRNA的重新配对介导的。与网织红细胞裂解物中的2.5倍相比，下游假结刺激移码30倍。当移位位点和假结之间的间隔区的长度延长三个核苷酸时，+1和-2移码变得相等。
Programmed translational frameshifting is essential for the expression of mammalian ornithine decarboxylase antizyme, a protein involved in the regulation of intracellular polyamines. A cassette containing antizyme frameshift signals is found to direct high-level (16%) frameshifting in yeast, Saccharomyces cerevisiae. In contrast to +1 frameshifting in the mammalian system, in yeast the same frame is reached by -2 frameshifting. Two bases are read twice. The -2 frameshifting is likely to be mediated by slippage of mRNA and re-pairing with the tRNA in the P-site. The downstream pseudoknot stimulates frameshifting by 30-fold compared with 2.5-fold in reticulocyte lysates. When the length of the spacer between the shift site and the pseudoknot is extended by three nucleotides, +1 and -2 frameshifting become equal.