Transient expression of β-galactosidase in differentiating sporozoites of Eimeria tenella
Transient expression of β-galactosidase in differentiating sporozoites of Eimeria tenella
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DOI：
10.1016/s0166-6851(98)00128-5
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发表时间：
1998-11-30
影响因子：
1.5
通讯作者：
Tomley, FM
中科院分区：
文献类型：
--
作者：
Kelleher, M;Tomley, FM
利用大肠杆菌中的β-半乳糖苷酶（β gal）作为报告酶，建立了一种针对顶复门Eimeria tenella的瞬时转染系统。成功表达的构建体含有E. tenella微线体基因Etmic-1融合到lacZ的编码区。表达β-gal的转染子能够侵入宿主细胞，并通过部分生命周期进行，形成裂殖子从其中释放的宿主。这表明，转染子至少可以分化为第一代转基因植株。然而，与未电穿孔的子孢子相比，这种分化延迟了约15小时。从转染的子孢子产生的一些裂殖子也表达β gal。这些结果为建立稳定的E. tenella，使用β gal作为报告酶。(C)1998年由Elsevier Science B. V.出版，版权所有。
A transient transfection system has been developed for a member of the Apicomplexa, Eimeria tenella, using beta-galactosidase (beta gal) from Escherichia coli as the reporter enzyme. Successfully expressed constructs contained sequences of the E. tenella microneme gene Etmic-1 fused to the coding region of lacZ. Transfectants expressing beta gal were able to invade host cells and proceed through part of the life-cycle, forming schizonts from which merozoites were released. This indicated that transfectants could differentiate at least to first generation schizonts. However, this differentiation was delayed compared with unelectroporated sporozoites by approximately 15 h. Some merozoites arising from transfected sporozoites also expressed beta gal. These results are encouraging for the development of a stable transfection system for E. tenella, using beta gal as a reporter enzyme. (C) 1998 Published by Elsevier Science B.V. All rights reserved.