VEGF-A/HGF induce Prox-1 expression in the chick embryo chorioallantoic membrane lymphatic vasculature
VEGF-A/HGF induce Prox-1 expression in the chick embryo chorioallantoic membrane lymphatic vasculature
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DOI：
10.1007/s10238-009-0085-6
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发表时间：
2010-09-01
影响因子：
4.6
通讯作者：
Ribatti, Domenico
中科院分区：
文献类型：
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作者：
Cimpean, Anca Maria;Seclaman, Edward;Ribatti, Domenico
血管内皮生长因子-A（VEGF-A）和肝细胞生长因子（HGF）是已知的在正常和病理条件下的血管生成诱导剂和促进剂。最近的数据表明，VEGF-A和HGF也可以影响淋巴管生成，但这一问题尚未完全阐明。VEGF-A和HGF联合给药已被用于改善不同实验模型中的血管生成反应，但它们对淋巴管生成的影响尚未研究。本研究的目的是表征VEGF-A/HGF给药的血液和淋巴管反应。为此，我们构建了适合于体内研究的pBlast VEGF-A/HGF组合。以鸡胚绒毛尿囊膜（CAM）法为体内实验模型，应用pBlast VEGF-A/HGF组合7天。结果显示VEGF-A/HGF联合应用能诱导CAM淋巴管内皮细胞产生强烈的血管生成反应，并诱导Prox-1的表达。有关的可能机制已被推测。
Vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF) are well known angiogenesis inductors and promoters in normal and pathologic conditions. Recent data showed that VEGF-A and HGF could also influence lymphangiogenesis but this matter has not been completely elucidated. Administration of VEGF-A and HGF in combination has been used to improve the angiogenic response in different experimental models, but their effects on lymphangiogenesis have not been investigated. The aim of this study was to characterize blood and lymphatic vascular response to VEGF-A/HGF administration. To this purpose, we built a pBlast VEGF-A/HGF combination suitable for in vivo research. By using as an experimental in vivo model the chick embryo chorioallantoic membrane (CAM) assay, we applied pBlast VEGF-A/HGF combination for 7 days. Results showed that VEGF-A/HGF combination was able to induce a strong angiogenic response and the expression of Prox-1 in the lymphatic endothelial cells of the CAM. The possible mechanisms involved have been speculated.