Isolation and characterization of 18 polymorphic microsatellite markers for the "Female Ginseng" Angelica sinensis (Apiaceae) and cross-species amplification
Isolation and characterization of 18 polymorphic microsatellite markers for the "Female Ginseng" Angelica sinensis (Apiaceae) and cross-species amplification
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DOI：
10.1016/j.bse.2015.07.013
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发表时间：
2015-08-01
影响因子：
1.6
通讯作者：
Huang, Luqi
中科院分区：
文献类型：
--
作者：
Lu, Youyuan;Cheng, Tao;Huang, Luqi
当归(Apiaceae)是一种濒危高山草本植物，在传统中药中被广泛应用。在中国，野生中华按蚊种群已经变得相当罕见。因此，迫切需要对该物种进行种群遗传学研究，以有效保护和可持续利用该物种。然而，到目前为止，还没有在中华绒毛虫中分离到微卫星座位。为了解决这个问题，我们分离了18个多态基因座，并对从6个群体收集的120个个体进行了基因分型。每个座位的等位基因数在1.2~5.5之间，平均为2.4。群体每个基因座的观测杂合度和预期杂合度分别为0.000~0.983(平均0.198)和0.066~0.661(平均0.333)。在不同群体中，有4到14个基因座偏离了Hardy-Weinberg平衡(p<；0.01)。这些微卫星标记在10种近缘植物中进行了交叉扩增，其中7个座位在所有物种中均被成功扩增。这些微卫星标记可用于遗传研究、中华按蚊的保存管理和中华按蚊的鉴定。(C)2015爱思唯尔有限公司。保留所有权利。
Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved.