Genetic Evidence for Transboundary Circulation of Peste Des Petits Ruminants Across West Africa
Genetic Evidence for Transboundary Circulation of Peste Des Petits Ruminants Across West Africa
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DOI：
10.3389/fvets.2019.00275
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发表时间：
2019-08-21
影响因子：
3.2
通讯作者：
Bataille, Arnaud
中科院分区：
文献类型：
--
作者：
Tounkara, Kadidia;Kwiatek, Olivier;Bataille, Arnaud
小反刍兽疫（PPR）是一种主要影响小型反刍动物的病毒性疾病。由于其跨界性质，控制战略的区域协调将是正在进行的根除PPR运动取得成功的关键。在这里，我们的目的是探讨在西非的PPR的跨界移动的程度，使用系统发育分析的基础上部分病毒基因序列。我们收集了样品，并获得部分核蛋白基因序列从西部和中部非洲的国家内的PPR感染的小反刍动物。将这一新的序列数据与该地区的生物学可用数据相结合，进行系统发育分析。在一个采样仍然很差的地区，总共获得了55个序列。系统发育分析表明，本研究中获得的大多数病毒序列都位于来自多个西非和中非国家的基因簇重组样本中。其中一些群组包含来自共享边界的国家的样本。在其他情况下，聚类将来自遥远国家的样本分组。我们的研究结果表明，广泛和经常性的PPR在西非境内的越境转移，支持需要在该地区的PPR监测和控制的区域协调战略。基于现有数据的简单系统发育分析可以提供PPR跨界动态的信息，因此，可能有助于改善控制策略。正在进行的和未来的PPR项目应包括广泛的遗传特征和流行病毒株的系统发育分析，以支持全球根除该疾病的运动。
Peste des Petits Ruminants (PPR) is a viral disease affecting predominantly small ruminants. Due to its transboundary nature, regional coordination of control strategies will be key to the success of the on-going PPR eradication campaign. Here, we aimed at exploring the extent of transboundary movement of PPR in West Africa using phylogenetic analyses based on partial viral gene sequences. We collected samples and obtained partial nucleoprotein gene sequence from PPR-infected small ruminants across countries within West and Central Africa. This new sequence data was combined with publically available data from the region to perform phylogenetic analyses. A total of fifty-five sequences were obtained in a region still poorly sampled. Phylogenetic analyses showed that the majority of virus sequences obtained in this study were placed within genetic clusters regrouping samples from multiple West African and Central African countries. Some of these clusters contained samples from countries sharing borders. In other cases, clusters grouped samples from very distant countries. Our results suggest extensive and recurrent transboundary movements of PPR within West Africa, supporting the need for a regional coordinated strategy for PPR surveillance and control in the region. Simple phylogenetic analyses based on readily available data can provide information on PPR transboundary dynamics and, therefore, could contribute to improve control strategies. On-going and future projects dedicated to PPR should include extensive genetic characterization and phylogenetic analyses of circulating viral strains in their effort to support the campaign for global eradication of the disease.