Nonintegrating Human Somatic Cell Reprogramming Methods.
Nonintegrating Human Somatic Cell Reprogramming Methods.
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DOI：
10.1007/10_2017_29
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
T. Schlaeger
中科院分区：
文献类型：
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作者：
T. Schlaeger
传统的生物医学研究和临床前研究经常依赖动物模型，并反复利用相对较小的一组人类细胞系，例如 HeLa、HEK293、HepG2、HL60 和 PANC1 细胞。然而，动物模型通常无法重现重要的临床表型，并且常规细胞系仅代表少数细胞类型或疾病，种族/遗传多样性非常有限，并且要么快速衰老，要么携带潜在的令人困惑的永生突变。近年来，人类多能干细胞引起了广泛关注，部分原因是这些细胞有望对人类疾病进行更精确的建模。人们对多能干细胞技术能够提供基于细胞的疗法来治疗各种退行性疾病和其他疾病也抱有很高的期望。本综述重点关注附加型和仙台病毒重编程方式，它们是从易于获取的细胞来源生成无转基因人类诱导多能干细胞 (hiPSC) 的最流行方法。图形摘要
Traditional biomedical research and preclinical studies frequently rely on animal models and repeatedly draw on a relatively small set of human cell lines, such as HeLa, HEK293, HepG2, HL60, and PANC1 cells. However, animal models often fail to reproduce important clinical phenotypes and conventional cell lines only represent a small number of cell types or diseases, have very limited ethnic/genetic diversity, and either senesce quickly or carry potentially confounding immortalizing mutations. In recent years, human pluripotent stem cells have attracted a lot of attention, in part because these cells promise more precise modeling of human diseases. Expectations are also high that pluripotent stem cell technologies can deliver cell-based therapeutics for the cure of a wide range of degenerative and other diseases. This review focuses on episomal and Sendai viral reprogramming modalities, which are the most popular methods for generating transgene-free human induced pluripotent stem cells (hiPSCs) from easily accessible cell sources.Graphical Abstract