Effects of chelating agent and environmental stresses on microbial biofilms: relevance to clinical microbiology
Effects of chelating agent and environmental stresses on microbial biofilms: relevance to clinical microbiology
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DOI：
10.1111/j.1365-2672.2011.04983.x
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发表时间：
2011-05-01
影响因子：
4
通讯作者：
Price, R. G.
中科院分区：
文献类型：
--
作者：
Al-Azemi, A.;Fielder, M. D.;Price, R. G.
目的：研究pH、温度、干燥、乙二胺四乙酸（EDTA）和去铁胺B（DFO）对杀白细胞素阳性的社区获得性甲氧西林敏感金黄色葡萄球菌（PVL + ve CA-MSSA）生物膜形成的影响。PVL +ve CA-MSSA比它们的渗透等同物更耐pH和热。干燥研究表明，PVL +ve CA-MSSA生物膜比脱水细胞更难处理。在1 mmol l-1 EDTA存在下观察到PVL +ve CA-MSSA生物膜形成的显著抑制。低浓度（2个中心点5 μ mol l-1）的DFO增强PVL +ve CA-MSSA生物膜的生长。在较高的浓度（1 mmol l-1），DFO抑制生长，但没有那么多的EDTA。EDTA和DFO的组合抑制PVL +VE CA-MSSA生物膜形成在较低的浓度比任何单独的Conclusions：这项研究表明，PVL +VE CA-MSSA生物膜是耐环境应力，但其生长可以有效地抑制由EDTA和DFO的混合物的研究的意义和影响：使用螯合剂的PVL +VE CA-MSSA的生物膜形成的抑制以前没有报道，并提供了一个实用的方法来实现这些潜在的重要的生物膜形成的新兴病原体的破坏。
Aims:To determine the effect of pH, temperature, desiccation, ethylenediaminetetraacetic acid (EDTA) and desferrioxamine B (DFO) on Panton-Valentine leukocidin-positive community acquired methicillin-susceptible Staphylococcus aureus (PVL +ve CA-MSSA) biofilm formation.Methods and Results:Biofilms from PVL +ve CA-MSSA (clinical isolate) were subjected to pH, temperature, desiccation, EDTA and DFO. PVL +ve CA-MSSA were more resistant to pH and heat than their planktonic equivalents. Desiccation studies demonstrated that PVL +ve CA-MSSA biofilms were more refractory to the treatment than planktonic cells. Significant inhibition of PVL +ve CA-MSSA biofilm formation was observed in the presence of 1 mmol l-1 EDTA. Low concentrations (2 center dot 5 mu mol l-1) of DFO enhanced the growth of PVL +ve CA-MSSA biofilms. At higher concentrations (1 mmol l-1), DFO did inhibit the growth but not as much as EDTA. A combination of EDTA and DFO inhibited PVL +ve CA-MSSA biofilm formation at lower concentrations than either alone.Conclusions:This study demonstrates that PVL +ve CA-MSSA biofilms are resistant to environmental stress but their growth can inhibited effectively by a mixture of EDTA and DFO.Significance and Impact of the Study:The inhibition of biofilm formation by PVL +ve CA-MSSA using chelating agents has not been previously reported and provides a practical approach to achieve the disruption of these potentially important biofilms formed by an emerging pathogen.