Molecular identification and anti-pathogenic activities of putative probiotic bacteria isolated from faeces of healthy elderly individuals
Molecular identification and anti-pathogenic activities of putative probiotic bacteria isolated from faeces of healthy elderly individuals
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从健康老年人粪便中分离的推定益生菌的分子鉴定和抗病原活性
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
R. Rastall
中科院分区：
文献类型：
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作者：
E. Likotrafiti;K. Manderson;F. Fava;K. Tuohy;G. Gibson;R. Rastall
从65岁健康老年人粪便标本中分离到109株乳酸菌，其中类双歧杆菌56株，类乳杆菌53株。通过表型分析(API)和16S rDNA测序对分离株进行种水平鉴定。共鉴定出乳杆菌11种，双歧杆菌6种。16S rDNA序列比对显示，发酵乳杆菌和双歧杆菌与婴儿双歧杆菌关系密切。对分离出的菌株进行了对艰难梭菌、肠致病性大肠埃希菌(EPEC)、产胞毒素大肠埃希菌(VTEC)和空肠弯曲杆菌的抗菌活性测定。乳酸菌的抗菌活性各不相同，少数菌株对所有病原菌都有抑制活性。双歧杆菌对空肠弯曲菌和氯离子有较高的抑制活性。比对大肠杆菌菌株更难耐药。关键词：乳杆菌，双歧杆菌，老年人，胃肠道微生物区系，抑制，艰难梭菌，致病性肠杆菌，产胞毒素大肠杆菌，空肠弯曲菌。
One hundred and nine lactic acid bacterial strains (56 bifidobacteria-like and 53 lactobacilli-like) were isolated from faecal samples donated by healthy elderly individuals (>65 years old). Isolates were identified to species level by phenotypic analysis (by API) and by 16S rDNA sequencing. Eleven species of Lactobacillus and six species of Bifidobacterium were identified. The most frequently isolated lactobacillus was L. fermentum and the most frequently isolated bifidobacterium was closely related to B. infantis by 16S rDNA sequence alignment. The isolates were characterized for their antimicrobial activity against Clostridium difficile, enteropathogenic Escherichia coli (EPEC), verocytotoxigenic E. coli (VTEC) and Campylobacter jejuni. The lactobacilli displayed variations in their antimicrobial activity with few strains showing inhibitory activity against all pathogens. The bifidobacteria displayed higher levels of inhibitory activity against C. jejuni and Cl. difficile than against the E. coli strains. Keywords: Lactobacillus, Bifidobacterium, elderly, gastrointestinal microbiota, inhibition, Clostridium difficile, enteropathogenic Escherichia coli (EPEC), verocytotoxigenic E. coli (VTEC), Campylobacter jejuni.