Combined microextraction by packed sorbent and high-performance liquid chromatography-ultraviolet detection for rapid analysis of ractopamine in porcine muscle and urine samples
Combined microextraction by packed sorbent and high-performance liquid chromatography-ultraviolet detection for rapid analysis of ractopamine in porcine muscle and urine samples
复制标题
填充吸附剂微萃取与高效液相色谱-紫外检测相结合快速分析猪肌肉和尿液样品中的莱克多巴胺
DOI：
10.1016/j.foodchem.2013.08.094
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发表时间：
2014-02-15
期刊：
影响因子：
8.8
通讯作者：
Chang, Chun
中科院分区：
文献类型：
--
作者：
Du, Wei;Zhao, Gang;Chang, Chun
A method for rapid analysis of ractopamine in porcine muscle and urine was developed and validated. The method was based on combined of microextraction by packed sorbent (MEPS) and high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Parameters of the MEPS procedure affecting extraction efficiency were optimised. Optimum extraction conditions were 100 mu L of sample in five extraction cycles and sampling time of 0.9985). Accuracies of muscle and urine analyses were 93.9-109.2% and 93.4-105.1%, respectively. Intra-day and inter-day precisions (RSD%) were lower than 11.8% for both analyses. The method was applied for rapid analysis of ractopamine in biological samples. The method was simpler and could be used to screen other beta(2)-agonists in other extraction media. (C) 2013 Elsevier Ltd. All rights reserved.
