Silver Nanoparticles Addition in Poly(Methyl Methacrylate) Dental Matrix: Topographic and Antimycotic Studies
Silver Nanoparticles Addition in Poly(Methyl Methacrylate) Dental Matrix: Topographic and Antimycotic Studies
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DOI：
10.3390/ijms20194691
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Rinaldi, Rosaria
中科院分区：
文献类型：
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作者：
De Matteis, Valeria;Cascione, Mariafrancesca;Rinaldi, Rosaria
纳米粒子由于其独特的物理化学性质，在医疗器械中的广泛应用，为治疗和预防许多疾病和感染开辟了新的领域。通过这种方式，银纳米颗粒（AgNPs）即使在低浓度下也具有很强的抗菌活性，这是由于它们有选择性破坏细胞膜的能力。特别是在口腔医学领域，在不同种类的口腔修复基质中使用AgNPs可能是治疗免疫抑制患者遭受不同口腔感染的基本工具。白色念珠菌（C. albicans）是一种具有高度定植能力的机会致病性酵母菌，是口腔感染的病原体之一。在我们的工作中，我们在聚甲基丙烯酸甲酯（PMMA）中添加了两种浓度（3 wt%和3.5 wt%）的单分散柠檬酸盐覆盖AgNPs，其尺寸为20 nm，聚甲基丙烯酸甲酯（PMMA）是用于开发牙科修复体的常用树脂。在AgNPs表征后，我们通过原子力显微镜（AFM）评估了添加AgNPs后PMMA和PMMA的形貌改变，显示表面粗糙度降低。采用Miles和Misra技术以及扫描电镜（SEM）在暴露AgNPs后24 h和48 h对白色念珠菌在PMMA表面的定殖进行了评估，结果显示AgNPs暴露后酵母菌活力降低。
The widespread use of nanoparticles (NPs) in medical devices has opened a new scenario in the treatment and prevention of many diseases and infections owing to unique physico-chemical properties of NPs. In this way, silver nanoparticles (AgNPs) are known to have a strong antimicrobial activity, even at low concentrations, due to their ability to selectively destroy cellular membranes. In particular, in the field of dental medicine, the use of AgNPs in different kinds of dental prosthesis matrixes could be a fundamental tool in immunodepressed patients that suffer of different oral infections. Candida albicans (C. albicans), an opportunistic pathogenic yeast with high colonization ability, is one of the causative agents of oral cavity infection. In our work, we added monodispersed citrate-capping AgNPs with a size of 20 nm at two concentrations (3 wt% and 3.5 wt%) in poly(methyl methacrylate) (PMMA), the common resin used to develop dental prostheses. After AgNPs characterization, we evaluated the topographical modification of PMMA and PMMA with the addition of AgNPs by means of atomic force microscopy (AFM), showing the reduction of surface roughness. The C. albicans colonization on PMMA surfaces was assessed by the Miles and Misra technique as well as by scanning electron microscopy (SEM) at 24 h and 48 h with encouraging results on the reduction of yeast viability after AgNPs exposure.